Characterization of a para-nitrophenol catabolic cluster in pseudomonas sp strain nyz402 and construction of an engineered strain capable of simultaneously mineralizing both para- and ortho-nitrophenols
文献类型：期刊论文
| 作者 | Wei, Qing; Liu, Hong; Zhang, Jun-Jie; Wang, Song-He; Xiao, Yi; Zhou, Ning-Yi1 |
| 刊名 | Biodegradation
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 21期号:4页码:575-584 |
| ISSN号 | 0923-9820 |
| 关键词 | Catabolism Engineered strain Ortho-nitrophenol Pseudomonas Para-nitrophenol Simultaneous degradation |
| DOI | 10.1007/s10532-009-9325-4 |
| 通讯作者 | Zhou, ning-yi(n.zhou@pentium.whiov.ac.cn) |
| 英文摘要 | Pseudomonas sp. strain nyz402 was isolated for its ability to grow on para-nitrophenol (pnp) as a sole source of carbon, nitrogen, and energy, and was shown to degrade pnp via an oxidization pathway. this strain was also capable of growing on hydroquinone or catechol. a 15, 818 bp dna fragment extending from a 800-bp dna fragment of hydroxyquinol 1,2-dioxygenase gene (pnpg) was obtained by genome walking. sequence analysis indicated that the pnp catabolic gene cluster (pnpabcdefg) in this fragment shared significant similarities with a recently reported gene cluster responsible for pnp degradation from pseudomonas sp. strain wbc-3. pnpa is pnp 4-monooxygenase converting pnp to hydroquinone via benzoquinone in the presence of nadph, and genetic analysis indicated that pnpa plays a key role in pnp degradation. pnpa1 present in the upstream of the cluster (absent in the cluster from strain wbc-3) encodes a protein sharing as high as 55% identity with pnpa, but was not involved in pnp degradation by either in vitro or in vivo analyses. furthermore, an engineered strain capable of growing on pnp and ortho-nitrophenol (onp) was constructed by introducing onpab (encoding onp monooxygenase and ortho-benzoquinone reductase which catalyzed the transformation of onp to catechol) from alcaligenes sp. strain nyz215 into strain nyz402. |
| WOS关键词 | P-NITROPHENOL ; GENE-CLUSTER ; ARTHROBACTER SP ; BIODEGRADATION ; PUTIDA ; MONOOXYGENASE ; DEGRADATION ; PLASMID ; CLONING ; SYSTEM |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000278087900007 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375679 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, Ning-Yi |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Appl & Environm Microbiol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Qing,Liu, Hong,Zhang, Jun-Jie,et al. Characterization of a para-nitrophenol catabolic cluster in pseudomonas sp strain nyz402 and construction of an engineered strain capable of simultaneously mineralizing both para- and ortho-nitrophenols[J]. Biodegradation,2010,21(4):575-584. |
| APA | Wei, Qing,Liu, Hong,Zhang, Jun-Jie,Wang, Song-He,Xiao, Yi,&Zhou, Ning-Yi.(2010).Characterization of a para-nitrophenol catabolic cluster in pseudomonas sp strain nyz402 and construction of an engineered strain capable of simultaneously mineralizing both para- and ortho-nitrophenols.Biodegradation,21(4),575-584. |
| MLA | Wei, Qing,et al."Characterization of a para-nitrophenol catabolic cluster in pseudomonas sp strain nyz402 and construction of an engineered strain capable of simultaneously mineralizing both para- and ortho-nitrophenols".Biodegradation 21.4(2010):575-584. |
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来源：武汉病毒研究所
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