Para-nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in pseudomonas sp strain wbc-3
文献类型：期刊论文
| 作者 | Wei, Min1,2; Zhang, Jun-Jie1,2; Liu, Hong1,2; Zhou, Ning-Yi1,2 |
| 刊名 | Biodegradation
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 21期号:6页码:915-921 |
| 关键词 | 4-nitrocatechol Hydroxyquinol 1,2-dioxygenase Para-nitrophenol 4-monooxygenase Pseudomonas Transformation |
| ISSN号 | 0923-9820 |
| DOI | 10.1007/s10532-010-9351-2 |
| 通讯作者 | Zhou, ning-yi(n.zhou@pentium.whiov.ac.cn) |
| 英文摘要 | Pseudomonas sp. strain wbc-3 utilizes para-nitrophenol (pnp) as a sole source of carbon, nitrogen and energy. pnpa (pnp 4-monooxygenase) and pnpb (para-benzoquinone reductase) were shown to be involved in the initial steps of pnp catabolism via hydroquinone. we demonstrated here that pnpa also catalyzed monooxygenation of 4-nitrocatechol (4-nc) to hydroxyquinol, probably via hydroxyquinone. it was the first time that a single-component pnp monooxygenase has been shown to catalyze this conversion. pnpg encoded by a gene located in the pnp degradation cluster was purified as a his-tagged protein and identified as a hydroxyquinol dioxygenase catalyzing a ring-cleavage reaction of hydroxyquinol. although all the genes necessary for 4-nc metabolism seemed to be present in the pnp degradation cluster in strain wbc-3, it was unable to grow on 4-nc as a sole source of carbon, nitrogen and energy. this was apparently due to the substrate's inability to trigger the expression of genes involved in degradation. nevertheless, strain wbc-3 could completely degrade both pnp and 4-nc when pnp was used as the inducer, demonstrating its potential in bioremediation of the environment polluted by both 4-nc and pnp. |
| WOS关键词 | P-NITROPHENOL ; BURKHOLDERIA-CEPACIA ; ESCHERICHIA-COLI ; METHYL PARATHION ; CATABOLISM ; PATHWAY ; IDENTIFICATION ; OXIDOREDUCTASE ; DEGRADATION ; DIOXYGENASE |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000283153200006 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375684 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, Ning-Yi |
| 作者单位 | 1.Wuhan Inst Virol, Chinese Acad Sci, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Min,Zhang, Jun-Jie,Liu, Hong,et al. Para-nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in pseudomonas sp strain wbc-3[J]. Biodegradation,2010,21(6):915-921. |
| APA | Wei, Min,Zhang, Jun-Jie,Liu, Hong,&Zhou, Ning-Yi.(2010).Para-nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in pseudomonas sp strain wbc-3.Biodegradation,21(6),915-921. |
| MLA | Wei, Min,et al."Para-nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in pseudomonas sp strain wbc-3".Biodegradation 21.6(2010):915-921. |
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来源：武汉病毒研究所
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