Characterization and mutagenesis of a two-component monooxygenase involved in para-nitrophenol degradation by an arthrobacter strain
文献类型：期刊论文
| 作者 | Liu, Ping-Ping1,2; Zhang, Jun-Jie1; Zhou, Ning-Yi1 |
| 刊名 | International biodeterioration & biodegradation
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 64期号:4页码:293-299 |
| 关键词 | Arthrobacter sp strain nyz415 Para-benzoquinone Para-nitrophenol monooxygenase Site-directed mutagenesis |
| ISSN号 | 0964-8305 |
| DOI | 10.1016/j.ibiod.2010.03.001 |
| 通讯作者 | Zhou, ning-yi(n.zhou@pentium.whiov.ac.cn) |
| 英文摘要 | Para-nitrophenol (pnp) degradation cluster was cloned from a newly isolated arthrobacter sp. strain nyz415 by genome walking extending from a conserved region of gene encoding the oxygenase component of pnp monooxygenase. sequence analysis indicated it contained 12 open reading frames including genes npda1 and npda2 which encoded the reductase and oxygenase components of pnp monooxygenase, respectively. both npda1 and npda2 were expressed and purified to homogeneity. hydroquinone and hydroxyquinol were captured when pnp was transformed by purified npda1a2 and para-benzoquinone was also captured for the first time as one of the products of pnp transformation by monooxygenase involved in the hydroxyquinol pathway. sequence analysis showed four conserved residues (arg100, gln158, arg161 and thr193) related to the binding between the oxygenase component and substrate. substitutions of any of above four residues to ala have resulted in the complete loss of its activity on pnp transformation, indicating these four residues play important roles in the catalytic activity of pnp monooxygenase. (c) 2010 elsevier ltd. all rights reserved. |
| WOS关键词 | P-NITROPHENOL ; GENE-CLUSTER ; 4-NITROPHENOL HYDROXYLASE ; SEQUENCE ALIGNMENT ; METHYL PARATHION ; BIODEGRADATION ; CATABOLISM ; OXIDATION ; PLASMID ; CLONING |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology |
| WOS类目 | Biotechnology & Applied Microbiology ; Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000278787100005 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375686 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, Ning-Yi |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ping-Ping,Zhang, Jun-Jie,Zhou, Ning-Yi. Characterization and mutagenesis of a two-component monooxygenase involved in para-nitrophenol degradation by an arthrobacter strain[J]. International biodeterioration & biodegradation,2010,64(4):293-299. |
| APA | Liu, Ping-Ping,Zhang, Jun-Jie,&Zhou, Ning-Yi.(2010).Characterization and mutagenesis of a two-component monooxygenase involved in para-nitrophenol degradation by an arthrobacter strain.International biodeterioration & biodegradation,64(4),293-299. |
| MLA | Liu, Ping-Ping,et al."Characterization and mutagenesis of a two-component monooxygenase involved in para-nitrophenol degradation by an arthrobacter strain".International biodeterioration & biodegradation 64.4(2010):293-299. |
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来源：武汉病毒研究所
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