Calnexin inhibits thermal aggregation and neurotoxicity of prion protein
文献类型：期刊论文
| 作者 | Wang, Wenxi2,3; Chen, Rui2,3; Luo, Kan2,3; Wu, Di2,3; Huang, Liqin2,3; Huang, Tao2,3; Xiao, Gengfu1,2,3 |
| 刊名 | Journal of cellular biochemistry
 |
| 出版日期 | 2010-10-01 |
| 卷号 | 111期号:2页码:343-349 |
| 关键词 | Prion Calnexin Molecular chaperone Cytotoxicity Apoptosis |
| ISSN号 | 0730-2312 |
| DOI | 10.1002/jcb.22698 |
| 通讯作者 | Xiao, gengfu(xiaogf@wh.iov.cn) |
| 英文摘要 | Prion diseases are fatal neurodegenerative disorder associated with the conversion of the cellular isoform of the prion protein (prp(c)) into the infectious scrapie isoform (prp(sc)). deposition of misfolded prion proteins (prp) on certain regions of brain can result in prion diseases. as a membrane-bound chaperone of the endoplasmic reticulum (er), calnexin ensures the proper folding and quality control of newly synthesized proteins. using purified components in vitro, calnexin associated with many proteins and suppresses their thermal aggregation effectively. we for the first time analyzed prp-calnexin interaction. the immunoprecipitation, confocal microscope and native polyacrylamide-gel electrophoresis results indicated that calnexin could bind prp both in vitro and in vivo. the turbidity result showed that calnexin could supress thermal aggregation of prp. mtt, flow cytometry (fcm) and caspase activity studies demonstrated that calnexin prevent caspase-3-mediated cytotoxicity induced by prp. these results implied that calnexin is potentially beneficial for the resistance of prion diseases. j. cell. biochem. 111: 343-349, 2010. (c) 2010 wiley-liss, inc. |
| WOS关键词 | ENDOPLASMIC-RETICULUM ; QUALITY-CONTROL ; MOLECULAR CHAPERONE ; IN-VITRO ; DEGRADATION ; CALRETICULIN ; DISEASES ; NEURODEGENERATION ; OLIGOSACCHARIDE ; GLYCOPROTEINS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000282482400010 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375688 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xiao, Gengfu |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Modern Virol Res Ctr, Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wenxi,Chen, Rui,Luo, Kan,et al. Calnexin inhibits thermal aggregation and neurotoxicity of prion protein[J]. Journal of cellular biochemistry,2010,111(2):343-349. |
| APA | Wang, Wenxi.,Chen, Rui.,Luo, Kan.,Wu, Di.,Huang, Liqin.,...&Xiao, Gengfu.(2010).Calnexin inhibits thermal aggregation and neurotoxicity of prion protein.Journal of cellular biochemistry,111(2),343-349. |
| MLA | Wang, Wenxi,et al."Calnexin inhibits thermal aggregation and neurotoxicity of prion protein".Journal of cellular biochemistry 111.2(2010):343-349. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。