Duckweed (lemna minor) as a model plant system for the study of human microbial pathogenesis
文献类型：期刊论文
| 作者 | Zhang, Yong1; Hu, Yangbo1,3; Yang, Baoyu1; Ma, Fang1,3; Lu, Pei1,3; Li, Lamei1,3; Wan, Chengsong2; Rayner, Simon1; Chen, Shiyun1 |
| 刊名 | Plos one
 |
| 出版日期 | 2010-10-25 |
| 卷号 | 5期号:10页码:11 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0013527 |
| 通讯作者 | Zhang, yong() |
| 英文摘要 | Background: plant infection models provide certain advantages over animal models in the study of pathogenesis. however, current plant models face some limitations, e. g., plant and pathogen cannot co-culture in a contained environment. development of such a plant model is needed to better illustrate host-pathogen interactions. methodology/principal findings: we describe a novel model plant system for the study of human pathogenic bacterial infection on a large scale. this system was initiated by co-cultivation of axenic duckweed (lemna minor) plants with pathogenic bacteria in 24-well polystyrene cell culture plate. pathogenesis of bacteria to duckweed was demonstrated with pseudomonas aeruginosa and staphylococcus aureus as two model pathogens. p. aeruginosa pao1 caused severe detriment to duckweed as judged from inhibition to frond multiplication and chlorophyll formation. using a gfp-marked pao1 strain, we demonstrated that bacteria colonized on both fronds and roots and formed biofilms. virulence of pao1 to duckweed was attenuated in its quorum sensing (qs) mutants and in recombinant strains overexpressing the qs quenching enzymes. rn4220, a virulent strain of s. aureus, caused severe toxicity to duckweed while an avirulent strain showed little effect. using this system for antimicrobial chemical selection, green tea polyphenols exhibited inhibitory activity against s. aureus virulence. this system was further confirmed to be effective as a pathogenesis model using a number of pathogenic bacterial species. conclusions/significance: our results demonstrate that duckweed can be used as a fast, inexpensive and reproducible model plant system for the study of host-pathogen interactions, could serve as an alternative choice for the study of some virulence factors, and could also potentially be used in large-scale screening for the discovery of antimicrobial chemicals. |
| WOS关键词 | PSEUDOMONAS-AERUGINOSA VIRULENCE ; RESISTANT STAPHYLOCOCCUS-AUREUS ; CAENORHABDITIS-ELEGANS ; ARABIDOPSIS-THALIANA ; BIOFILM FORMATION ; INFECTION MODEL ; INNATE IMMUNITY ; PATHOGENICITY ; HOST ; ANIMALS |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000283422100005 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375711 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Yong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China 2.So Med Univ, Sch Publ Hlth & Trop Med, Dept Microbiol, Guangzhou, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yong,Hu, Yangbo,Yang, Baoyu,et al. Duckweed (lemna minor) as a model plant system for the study of human microbial pathogenesis[J]. Plos one,2010,5(10):11. |
| APA | Zhang, Yong.,Hu, Yangbo.,Yang, Baoyu.,Ma, Fang.,Lu, Pei.,...&Chen, Shiyun.(2010).Duckweed (lemna minor) as a model plant system for the study of human microbial pathogenesis.Plos one,5(10),11. |
| MLA | Zhang, Yong,et al."Duckweed (lemna minor) as a model plant system for the study of human microbial pathogenesis".Plos one 5.10(2010):11. |
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来源：武汉病毒研究所
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