New regulatory mechanisms for the intracellular localization and trafficking of influenza a virus ns1 protein revealed by comparative analysis of a/pr/8/34 and a/sydney/5/97
文献类型：期刊论文
| 作者 | Han, Han1,2; Cui, Zong-Qiang1; Wang, Wei1,2; Zhang, Zhi-Ping1; Wei, Hong-Ping1; Zhou, Ya-Feng1; Zhang, Xian-En1 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 91页码:2907-2917 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir.0.024943-0 |
| 通讯作者 | Zhang, xian-en() |
| 英文摘要 | During influenza a virus infection, the ns1 protein is engaged in different functions in different intracellular compartments in this study, we showed that the ns1 of a/pr/8/34 localized in different positions from that of a/sydney/5/97 when transiently expressed in madin-darby canine kidney cells residue 221 of ns1 was identified to be a new key residue involved in the c-terminal nuclear localization signal (nls) and nucleolar localization signal (nols) of ns1 from a/sydney/5/97 analysis of chimeric ns1 and further mutants showed that residues responsible for the binding between ns1 and the cleavage and polyadenylation specificity factor (cpsf) are correlated with the intracellular localization of transiently expressed ns1 proteins fluorescence loss in photobleaching imaging revealed that the ns1 protein with both functional nlss and nuclear export signal (nes) was able to shuttle between the nucleus and cytoplasm drug inhibition experiments and fluorescence resonance energy transfer analysis suggested that ns1 was exported out of the cell nuclei via a crm1-independent pathway moreover, it is likely that another cytoplasmic localization-related sequence exists in the ns1 protein other than the leucine-rich nes these findings provide new insights into the mechanism of intracellular localization and trafficking of influenza a virus ns1 protein, which is important for understanding its function |
| WOS关键词 | NUCLEAR EXPORT SIGNAL ; PRE-MESSENGER-RNAS ; STRUCTURAL BASIS ; EFFECTOR DOMAIN ; IN-VIVO ; CRM1 ; COMPLEX ; INTERACTS ; RECEPTOR ; PATHWAY |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000285221000003 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375715 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Han,Cui, Zong-Qiang,Wang, Wei,et al. New regulatory mechanisms for the intracellular localization and trafficking of influenza a virus ns1 protein revealed by comparative analysis of a/pr/8/34 and a/sydney/5/97[J]. Journal of general virology,2010,91:2907-2917. |
| APA | Han, Han.,Cui, Zong-Qiang.,Wang, Wei.,Zhang, Zhi-Ping.,Wei, Hong-Ping.,...&Zhang, Xian-En.(2010).New regulatory mechanisms for the intracellular localization and trafficking of influenza a virus ns1 protein revealed by comparative analysis of a/pr/8/34 and a/sydney/5/97.Journal of general virology,91,2907-2917. |
| MLA | Han, Han,et al."New regulatory mechanisms for the intracellular localization and trafficking of influenza a virus ns1 protein revealed by comparative analysis of a/pr/8/34 and a/sydney/5/97".Journal of general virology 91(2010):2907-2917. |
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来源：武汉病毒研究所
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