Specificity of baculovirus p6.9 basic dna-binding proteins and critical role of the c terminus in virion formation
文献类型：期刊论文
| 作者 | Wang, Manli1,2,3; Tuladhar, Era1; Shen, Shu2,3; Wang, Hualin2,3; van Oers, Monique M.1; Vlak, Just M.1; Westenberg, Marcel1,4 |
| 刊名 | Journal of virology
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 84期号:17页码:8821-8828 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.00072-10 |
| 通讯作者 | Vlak, just m.(just.vlak@wur.nl) |
| 英文摘要 | The majority of double-stranded dna (dsdna) viruses infecting eukaryotic organisms use host- or virus-expressed histones or protamine-like proteins to condense their genomes. in contrast, members of the baculoviridae family use a protamine-like protein named p6.9. the dephosphorylated form of p6.9 binds to dna in a non-sequence-specific manner. by using a p6.9-null mutant of autographa californica multiple nucleopolyhedrovirus (acmnpv), we demonstrate that p6.9 is not required for viral dna replication but is essential for the production of infectious virus. virion production was rescued by p6.9 homologs from a number of alphabaculovirus species and one gammabaculovirus species but not from the genus betabaculovirus, comprising the granuloviruses, or by the p6.9 homolog vp15 from the unrelated white spot syndrome virus of shrimp. mutational analyses demonstrated that acmnpv p6.9 with a conserved 11-residue deletion of the c terminus was not capable of rescuing p6.9-null acmnpv, while a chimeric betabaculovirus p6.9 containing the p6.9 c-terminal region of an alphabaculovirus strain was able to do so. this implies that the c terminus of baculovirus p6.9 contains sequence elements essential for virion formation. such elements may possibly interact with species- or genus-specific domains of other nucleocapsid proteins during virus assembly. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; ENVELOPE FUSION PROTEIN ; INFECTING PLODIA-INTERPUNCTELLA ; SPOT SYNDROME VIRUS ; GRANULOSIS-VIRUS ; GENOME SEQUENCE ; NUCLEOCAPSID PROTEIN ; ESCHERICHIA-COLI ; PURIFIED CAPSIDS ; NUCLEOPOLYHEDROVIRUS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000280605300041 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375721 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Vlak, Just M. |
| 作者单位 | 1.Wageningen Univ, Virol Lab, NL-6708 PB Wageningen, Netherlands 2.Chinese Acad Sci, State Key Lab Virol, Key Lab Mol Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 4.Kings Coll London, Pharmaceut Sci Res Div, London SE1 9NH, England |
| 推荐引用方式 GB/T 7714 | Wang, Manli,Tuladhar, Era,Shen, Shu,et al. Specificity of baculovirus p6.9 basic dna-binding proteins and critical role of the c terminus in virion formation[J]. Journal of virology,2010,84(17):8821-8828. |
| APA | Wang, Manli.,Tuladhar, Era.,Shen, Shu.,Wang, Hualin.,van Oers, Monique M..,...&Westenberg, Marcel.(2010).Specificity of baculovirus p6.9 basic dna-binding proteins and critical role of the c terminus in virion formation.Journal of virology,84(17),8821-8828. |
| MLA | Wang, Manli,et al."Specificity of baculovirus p6.9 basic dna-binding proteins and critical role of the c terminus in virion formation".Journal of virology 84.17(2010):8821-8828. |
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来源：武汉病毒研究所
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