Improved purification and some properties of a novel phospholipase c from bacillus mycoides strain 970
文献类型：期刊论文
| 作者 | Wang, Chang Gao1,2; Chen, Ming Kai3; Chen, Tao1 |
| 刊名 | African journal of microbiology research
 |
| 出版日期 | 2010-03-04 |
| 卷号 | 4期号:5页码:396-399 |
| 关键词 | Phospholipase c Purification Metalloenzyme Nonhemolytic activity Bacillus mycoides |
| ISSN号 | 1996-0808 |
| 通讯作者 | Wang, chang gao(wcg71415@163.com) |
| 英文摘要 | A novel microbial phospholipase c from bacillus mycoides strain 970 was purified to apparent homogeneity by an improved purification process, in which deae-cellulose adsorption was first used to remove bulk of dark brown impurities and then a combination of deae-cellulose ion-exchange, phenyl sepharose hydrophobic interaction and sephacryl s-100 gel filtration chromatography was used to separate the enzyme from remained contaminants. the enzyme appeared to be a single peptide of 75.1 kda on sds-page. the optimum temperature and ph of the enzyme were 60 degrees c and 7.0 - 7.5, respectively. the enzyme was stable at temperatures lower than 50 degrees c and ph 5 - 9.5. this purified phospholipase c was characterized as a metallophospholipase c and ca(2+), mg(2+), zn(2+), ba(2+) were essential for its plc activity. the enzyme showed no lecithinase activity on egg yolk agar and also no hemolytic activity on blood agar. |
| WOS关键词 | CLOSTRIDIUM-PERFRINGENS ; CRYSTAL-STRUCTURE ; PICHIA-PASTORIS ; NITROPHENYLPHOSPHORYLCHOLINE ; CEREUS ; ENZYME |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000276323100011 |
| 出版者 | ACADEMIC JOURNALS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375728 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Chang Gao |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Hubei Univ Technol, Minist Educ, Key Lab Fermentat Engn, Wuhan 430068, Peoples R China 3.Wuhan Univ, Renmin Hosp, Wuhan 430060, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chang Gao,Chen, Ming Kai,Chen, Tao. Improved purification and some properties of a novel phospholipase c from bacillus mycoides strain 970[J]. African journal of microbiology research,2010,4(5):396-399. |
| APA | Wang, Chang Gao,Chen, Ming Kai,&Chen, Tao.(2010).Improved purification and some properties of a novel phospholipase c from bacillus mycoides strain 970.African journal of microbiology research,4(5),396-399. |
| MLA | Wang, Chang Gao,et al."Improved purification and some properties of a novel phospholipase c from bacillus mycoides strain 970".African journal of microbiology research 4.5(2010):396-399. |
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来源：武汉病毒研究所
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