Genetic analysis of west nile virus containing a complete 3 ' csi rna deletion
文献类型：期刊论文
| 作者 | Zhang, Bo1; Dong, Hongping2; Ye, Hanqing1; Tilgner, Mark2; Shi, Pei-Yong2 |
| 刊名 | Virology
 |
| 出版日期 | 2010-12-20 |
| 卷号 | 408期号:2页码:138-145 |
| 关键词 | Flavivirus replication Rna cyclization Cis rna element |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2010.09.033 |
| 通讯作者 | Shi, pei-yong(pei_yong.shi@novartis.com) |
| 英文摘要 | We report a genetic interplay among three pairs of long-distance rna interactions that are involved in west nile virus (wnv) genome cyclization and replication: 5'cs/3'csi (conserved sequence), 5'uar/3'uar (upstream aug region), and 5'dar/3'dar (downstream aug region). deletion of the complete 3'csi element is lethal for wnv replication, but the replication of the 3'csi deletion virus could be rescued by second site mutations. functional analysis, using a genome-length rna and replicon, mapped the compensatory mutations to the 5'uar/3'uar and 5'dar/3'dar regions. biochemical analysis showed that the 3'csi deletion abolished the 5' and 3' rna interaction of the genome; the compensatory mutations could partially restore the 5' and 3' genome cyclization. these results demonstrate, for the first time, that a flavivirus without 3'csi could restore genome cyclization and viral replication through enhancement of the 5'uar/3'uar and 5'dar/3'dar interactions. (c) 2010 elsevier inc. all rights reserved. |
| WOS关键词 | YELLOW-FEVER VIRUS ; DENGUE VIRUS ; MOSQUITO-BORNE ; CYCLIZATION SEQUENCES ; SECONDARY STRUCTURE ; FUNCTIONAL-ANALYSIS ; GENOME CYCLIZATION ; FLAVIVIRUS GENOME ; VIRAL TRANSLATION ; TICK-BORNE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000284304400002 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375734 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shi, Pei-Yong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12208 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Bo,Dong, Hongping,Ye, Hanqing,et al. Genetic analysis of west nile virus containing a complete 3 ' csi rna deletion[J]. Virology,2010,408(2):138-145. |
| APA | Zhang, Bo,Dong, Hongping,Ye, Hanqing,Tilgner, Mark,&Shi, Pei-Yong.(2010).Genetic analysis of west nile virus containing a complete 3 ' csi rna deletion.Virology,408(2),138-145. |
| MLA | Zhang, Bo,et al."Genetic analysis of west nile virus containing a complete 3 ' csi rna deletion".Virology 408.2(2010):138-145. |
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来源：武汉病毒研究所
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