Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of helicoverpa armigera nucleopolyhedrovirus
文献类型：期刊论文
| 作者 | Peng, Ke; Wu, Minzhi; Deng, Fei; Song, Jingjiao; Dong, Chunsheng; Wang, Hualin; Hu, Zhihong1 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 91页码:659-670 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir.0.017103-0 |
| 通讯作者 | Hu, zhihong(huzh@wh.iov.cn) |
| 英文摘要 | The purpose of this study was to identify protein-protein interactions among the components of the occlusion-derived virus (odv) of helicoverpa armigera nucleopolyhedrovirus (hearnpv), a group 11 alphabaculovirus in the family baculoviridae. to achieve this, 39 selected genes of potential odv structural proteins were cloned and expressed in the gal4 yeast two-hybrid (y2h) system. the direct-cross y2h assays identified 22 interactions comprising 13 binary interactions [ha9-odv-ec43, odv-e56-38k, odv-e56-pif3, lef3-helicase, lef3-alkaline nuclease (an), gp41-38k, gp41-ha90, 38k-pif3,38k-pif2, vp80-ha100, odv-e66-pif3, odv-e66-pif2 and pif3-pif2] and nine self-associations (ie1, ha44, lef3, ha66, gp41, cg30, 38k, pif3 and p24). five of these interactions - lef3-helicase and lef3-an, and the self-associations of ie1, lef3 and 38k - have been reported previously in autographa californica multiple nucleopolyhedrovirus. as ha44 and ha100 were two newly identified odv proteins of group 11 viruses, their interactions were further confirmed. the self-association of ha44 was verified with a his pull-down assay and the interaction of vp80-ha100 was confirmed by a coimmunoprecipitation assay. a summary of the protein-protein interactions of baculoviruses reported so far, comprising 68 interactions with 45 viral proteins and five host proteins, is presented, which will facilitate our understanding of the molecular mechanisms of baculovirus infection. |
| WOS关键词 | CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS ; SINGLE NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS ; BOMBYX-MORI NUCLEOPOLYHEDROVIRUS ; NUCLEAR POLYHEDROSIS-VIRUS ; DNA-BINDING-PROTEIN ; BACULOVIRUS TRANSREGULATOR IE1 ; HELIOTHIS-VIRESCENS LARVAE ; ACMNPV EXONO AC141 ; STRUCTURAL PROTEIN ; MULTICAPSID NUCLEOPOLYHEDROVIRUS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000275587200009 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375738 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, Zhihong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Ke,Wu, Minzhi,Deng, Fei,et al. Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of helicoverpa armigera nucleopolyhedrovirus[J]. Journal of general virology,2010,91:659-670. |
| APA | Peng, Ke.,Wu, Minzhi.,Deng, Fei.,Song, Jingjiao.,Dong, Chunsheng.,...&Hu, Zhihong.(2010).Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of helicoverpa armigera nucleopolyhedrovirus.Journal of general virology,91,659-670. |
| MLA | Peng, Ke,et al."Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of helicoverpa armigera nucleopolyhedrovirus".Journal of general virology 91(2010):659-670. |
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来源：武汉病毒研究所
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