Applying high-resolution melting (hrm) technology to identify five commonly used artemisia species
文献类型：期刊论文
| 作者 | Song, Ming1; Li, Jingjian2; Xiong, Chao3; Liu, Hexia4; Liang, Junsong5,6 |
| 刊名 | Scientific reports
 |
| 出版日期 | 2016-10-04 |
| 卷号 | 6页码:7 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep34133 |
| 通讯作者 | Liang, junsong(ljsylu@163.com) |
| 英文摘要 | Many members of the genus artemisia are important for medicinal purposes with multiple pharmacological properties. often, these herbal plants sold on the markets are in processed forms so it is difficult to authenticate. routine testing and identification of these herbal materials should be performed to ensure that the raw materials used in pharmaceutical products are suitable for their intended use. in this study, five commonly used artemisia species included artemisia argyi, artemisia annua, artemisia lavandulaefolia, artemisia indica, and artemisia atrovirens were analyzed using high resolution melting (hrm) analysis based on the internal transcribed spacer 2 (its2) sequences. the melting profiles of the its2 amplicons of the five closely related herbal species are clearly separated so that they can be differentiated by hrm method. the method was further applied to authenticate commercial products in powdered. hrm curves of all the commercial samples tested are similar to the botanical species as labeled. these congeneric medicinal products were also clearly separated using the neighbor-joining (nj) tree. therefore, hrm method could provide an efficient and reliable authentication system to distinguish these commonly used artemisia herbal products on the markets and offer a technical reference for medicines quality control in the drug supply chain. |
| WOS关键词 | HERBAL MEDICINE IDENTIFICATION ; DNA-BARCODE ; PRODUCTS ; RENAISSANCE ; TAXONOMY ; LEAVES |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000384597900001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375745 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liang, Junsong |
| 作者单位 | 1.Wuhan Univ Technol, Sch Chem Engn, Wuhan 430070, Peoples R China 2.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China 3.Hubei Univ Chinese Med, Coll Pharm, Wuhan 430065, Peoples R China 4.Chinese Acad Sci, Guangxi Inst Bot, Guilin 541006, Peoples R China 5.Yulin Normal Univ, Coll Life Sci Technol, Yulin 537000, Peoples R China 6.Yulin Normal Univ, Cultivat Base Key Lab Conservat & Utilizat Rare &, Yulin 537000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Ming,Li, Jingjian,Xiong, Chao,et al. Applying high-resolution melting (hrm) technology to identify five commonly used artemisia species[J]. Scientific reports,2016,6:7. |
| APA | Song, Ming,Li, Jingjian,Xiong, Chao,Liu, Hexia,&Liang, Junsong.(2016).Applying high-resolution melting (hrm) technology to identify five commonly used artemisia species.Scientific reports,6,7. |
| MLA | Song, Ming,et al."Applying high-resolution melting (hrm) technology to identify five commonly used artemisia species".Scientific reports 6(2016):7. |
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来源：中国科学院大学
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