Expression, purification of the ul3 protein of herpes simplex virus type 1, and production of ul3 polyclonal antibody
文献类型：期刊论文
| 作者 | Lin, Fusen1; Ren, Xiaoming2; Guo, Hong1; Ding, Qiong1; Zheng, Alan C.1 |
| 刊名 | Journal of virological methods
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 166期号:1-2页码:72-76 |
| 关键词 | Herpes simplex virus type 1 (hsv-1) Ul3 protein Protein expression Protein purification Polyclonal antibody Immunofluorescent assay |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2010.02.022 |
| 通讯作者 | Zheng, alan c.(zheng.alan@hotmail.com) |
| 英文摘要 | Herpes simplex virus type 1 (hsv-1) is a common pathogen which causes infections of the mucocutaneous membranes. the ul3 protein belongs to a group of hsv-1 late proteins. to date, the function of the ul3 protein in cell culture, animal models, and natural infection is unknown. to investigate further the function of the ul3 protein, this study was undertaken to express the ul3 protein and raise a polyclonal antibody. the ul3 gene was cloned in the prokaryotic expression vector pet-28a (+) to yield pet-28a (+)-ul3. the his6-tagged ul3 protein was expressed in escherichia coli (e. coli) bl21 (de3) cells and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). after purification by nickel affinity chromatography and refolding, the recombinant protein was used to raise the anti-vu polyclonal antibody. western blot analysis demonstrated that the ul3 protein was recognized by the polyclonal antibody, and immunofluorescent assay also showed that the antibody was able to recognize the ul3 protein in the cells infected with hsv-1. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | OPEN READING FRAME ; LOCALIZATION ; PHOSPHOPROTEIN ; ENCODES ; BODIES ; GENOME ; GENES ; U(L)3 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000278029600011 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375750 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Alan C. |
| 作者单位 | 1.Chinese Acad Sci, Mol Virol & Viral Immunol Res Grp, Natl Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.China Agr Univ, Dept Anim Sci, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Fusen,Ren, Xiaoming,Guo, Hong,et al. Expression, purification of the ul3 protein of herpes simplex virus type 1, and production of ul3 polyclonal antibody[J]. Journal of virological methods,2010,166(1-2):72-76. |
| APA | Lin, Fusen,Ren, Xiaoming,Guo, Hong,Ding, Qiong,&Zheng, Alan C..(2010).Expression, purification of the ul3 protein of herpes simplex virus type 1, and production of ul3 polyclonal antibody.Journal of virological methods,166(1-2),72-76. |
| MLA | Lin, Fusen,et al."Expression, purification of the ul3 protein of herpes simplex virus type 1, and production of ul3 polyclonal antibody".Journal of virological methods 166.1-2(2010):72-76. |
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来源：武汉病毒研究所
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