Cross-protection against influenza virus infection by intranasal administration of m1-based vaccine with chitosan as an adjuvant
文献类型：期刊论文
| 作者 | Sui, Zhiwei1,4; Chen, Quanjiao1; Fang, Fang2; Zheng, Mei3; Chen, Ze1,2,3 |
| 刊名 | Vaccine
 |
| 出版日期 | 2010-11-10 |
| 卷号 | 28期号:48页码:7690-7698 |
| 关键词 | Influenza Intranasal administration Mucosal adjuvant Chitosan M1 protein |
| ISSN号 | 0264-410X |
| DOI | 10.1016/j.vaccine.2010.09.019 |
| 通讯作者 | Chen, ze(chenze2005@263.net) |
| 英文摘要 | The antigenic variation of influenza virus represents a major health problem, thus continuous efforts have been made to develop broad-spectrum vaccines against influenza virus. matrix protein 1 (m1) protein is highly conserved in all influenza a strains. in this study, m1 protein was efficiently expressed in escherichia coli (e. coli), then purified and used for immunization of balb/c mice by intranasal drip using chitosan as adjuvant. the m1 protein was administered intranasally to mice in combination with chitosan adjuvant twice at an interval of 3 weeks. three weeks after the second immunization, the mice were challenged with a lethal dose (5 x ld50) of a/chicken/jiangsu/7/2002 (h9n2) virus, pr8 (h1n1) virus and a/chicken/henan/12/2004 (h5n1) virus. the protective immunity of the vaccine was evaluated by determining the survival rates, residual lung virus titers, bodyweight, and the serum antibody titers of the mice. the results showed that nasal administration of 100 mu g m1 in combination with chitosan could not only completely protect the mice effectively against the challenge of the homologous virus but also protect 70% and 30% of the mice against the heterologous hi ni and h5n1 viruses, respectively. the study indicated that the m1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the m1 vaccine. (c) 2010 elsevier ltd. all rights reserved. |
| WOS关键词 | EXPRESSING PLASMID DNAS ; A VIRUS ; IMMUNOGLOBULIN-A ; HETEROTYPIC IMMUNITY ; ANTIBODY-RESPONSES ; HIV-1 REPLICATION ; SYSTEMIC ANTIBODY ; NASAL DELIVERY ; BALB/C MICE ; MUCOSAL |
| WOS研究方向 | Immunology ; Research & Experimental Medicine |
| WOS类目 | Immunology ; Medicine, Research & Experimental |
| 语种 | 英语 |
| WOS记录号 | WOS:000285432600015 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375753 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Ze |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 3.Shanghai Inst Biol Prod, Shanghai 200052, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sui, Zhiwei,Chen, Quanjiao,Fang, Fang,et al. Cross-protection against influenza virus infection by intranasal administration of m1-based vaccine with chitosan as an adjuvant[J]. Vaccine,2010,28(48):7690-7698. |
| APA | Sui, Zhiwei,Chen, Quanjiao,Fang, Fang,Zheng, Mei,&Chen, Ze.(2010).Cross-protection against influenza virus infection by intranasal administration of m1-based vaccine with chitosan as an adjuvant.Vaccine,28(48),7690-7698. |
| MLA | Sui, Zhiwei,et al."Cross-protection against influenza virus infection by intranasal administration of m1-based vaccine with chitosan as an adjuvant".Vaccine 28.48(2010):7690-7698. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。