Construction of bifunctional phage display for biological analysis and immunoassay
文献类型：期刊论文
| 作者 | Guo, Yongchao1; Liang, Xiaosheng1,2; Zhou, Yafeng1; Zhang, Zhiping1; Wei, Hongping1; Men, Dong1,2; Luo, Ming1,2; Zhang, Xian-En1 |
| 刊名 | Analytical biochemistry
 |
| 出版日期 | 2010 |
| 卷号 | 396期号:1页码:155-157 |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2009.08.026 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) |
| 英文摘要 | A phage display-based bifunctional display system was developed for simple and sensitive immunoassay. the resulting bifunctional phage could simultaneously display a few single-chain variable fragment (scfv) and many copies of the gold-binding peptide on its surface, thereby mediating antigen recognition and signal amplification. as a demonstration study, it was possible for bifunctional phage-based immunoassay to identify bacillus anthracis spores from other bacillus strains with detection sensitivity 10-fold higher than that of conventional phage enzyme-linked immunosorbent assay (elisa). this protocol may be applied to build other bifunctional phage clones for broad applications (e.g., immunoassay kits, affinity biosensors, biorecognition assays). (c) 2009 elsevier inc. all rights reserved. |
| WOS关键词 | BACILLUS-ANTHRACIS ; RAPID-DETECTION ; SYSTEM ; SELECTION ; PROTEIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000272406100023 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375754 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Yongchao,Liang, Xiaosheng,Zhou, Yafeng,et al. Construction of bifunctional phage display for biological analysis and immunoassay[J]. Analytical biochemistry,2010,396(1):155-157. |
| APA | Guo, Yongchao.,Liang, Xiaosheng.,Zhou, Yafeng.,Zhang, Zhiping.,Wei, Hongping.,...&Zhang, Xian-En.(2010).Construction of bifunctional phage display for biological analysis and immunoassay.Analytical biochemistry,396(1),155-157. |
| MLA | Guo, Yongchao,et al."Construction of bifunctional phage display for biological analysis and immunoassay".Analytical biochemistry 396.1(2010):155-157. |
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来源：武汉病毒研究所
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