Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells
文献类型：期刊论文
| 作者 | Luo, Min Hua1,2; Hannemann, Holger1; Kulkarni, Amit S.1; Schwartz, Philip H.3; O'Dowd, John M.1; Fortunato, Elizabeth A.1 |
| 刊名 | Journal of virology
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 84期号:7页码:3528-3541 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.02161-09 |
| 通讯作者 | Fortunato, elizabeth a.(lfort@uidaho.edu) |
| 英文摘要 | Congenital human cytomegalovirus (hcmv) infection is a leading cause of birth defects, largely manifested as central nervous system (cns) disorders. the principal site of manifestations in the mouse model is the fetal brain's neural progenitor cell (npc)-rich subventricular zone. our previous human npc studies found these cells to be fully permissive for hcmv and a useful in vitro model system. in continuing work, we observed that under culture conditions favoring maintenance of multipotency, infection caused npcs to quickly and abnormally differentiate. this phenotypic change required active viral transcription. whole-genome expression analysis found rapid downregulation of genes that maintain multipotency and establish npcs' neural identity. quantitative pcr, western blot, and immunofluorescence assays confirmed that the mrna and protein levels of four hallmark npc proteins (nestin, doublecortin, sex-determining homeobox 2, and glial fibrillary acidic protein) were decreased by hcmv infection. the decreases required active viral replication and were due, at least in part, to proteasomal degradation. our results suggest that hcmv infection causes in utero cns defects by inducing both premature and abnormal differentiation of npcs. |
| WOS关键词 | CENTRAL-NERVOUS-SYSTEM ; PLURIPOTENT STEM-CELLS ; LONGITUDINAL-FOLLOW-UP ; MURINE CYTOMEGALOVIRUS ; PRECURSOR CELLS ; SIGNALING PROTEIN ; NEURONAL DIFFERENTIATION ; PROTEASOME INHIBITOR ; AUTISTIC DISORDER ; DNA-REPLICATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000275307400037 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375758 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Fortunato, Elizabeth A. |
| 作者单位 | 1.Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Childrens Hosp Orange Cty, Res Inst, Orange, CA 92868 USA |
| 推荐引用方式 GB/T 7714 | Luo, Min Hua,Hannemann, Holger,Kulkarni, Amit S.,et al. Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells[J]. Journal of virology,2010,84(7):3528-3541. |
| APA | Luo, Min Hua,Hannemann, Holger,Kulkarni, Amit S.,Schwartz, Philip H.,O'Dowd, John M.,&Fortunato, Elizabeth A..(2010).Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells.Journal of virology,84(7),3528-3541. |
| MLA | Luo, Min Hua,et al."Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells".Journal of virology 84.7(2010):3528-3541. |
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来源：武汉病毒研究所
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