Functional interaction between mutl and 3 '-5 ' exonuclease x in escherichia coli
文献类型：期刊论文
| 作者 | Cheng, Fang1,3; Hou, Jian1; Chen, Yuan-Yuan1; Zhou, Ying1; Zhang, Hong-Tai1; Bi, Li-Jun1; Zhang, Xian-En2 |
| 刊名 | Archives of biochemistry and biophysics
 |
| 出版日期 | 2010-10-01 |
| 卷号 | 502期号:1页码:39-43 |
| 关键词 | Mutl Exox Ionic interaction Promotion mechanism |
| ISSN号 | 0003-9861 |
| DOI | 10.1016/j.abb.2010.07.011 |
| 通讯作者 | Bi, li-jun() |
| 英文摘要 | Exonuclease x is a 3'-5' distributive exonuclease that functions in dna recombination and repair it undergoes multiple rounds of binding, hydrolysis, and release to degrade long substrate molecules and thus is very inefficient. in order to identify a cofactor that elevates the excision activity of exox, we screened many proteins involved in repair and recombination we observed that mutl greatly promoted the exonuclease activity of exox, and then verified the interaction between mutl and exox using spr and far-western analysis this promotion is independent of atp and the dna-binding activity of mutt. we constructed two deletion mutants to analyze this interaction and its regulation of exox activity, and found that this functional interaction with exox is mainly due to ionic interactions with the n-terminus of mutl this adds a new role to mutt. and gives a clue to mutl's possible regulation on other dnaq family exonuclease members (c) 2010 elsevier inc. all rights reserved |
| WOS关键词 | DIRECTED MISMATCH REPAIR ; DNA-POLYMERASE-I ; ATP HYDROLYSIS ; PROTEINS ; BINDING ; RECOMBINATION ; RECA ; UVRD ; VSR |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000281718000006 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375762 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Bi, Li-Jun |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol & Prote Platform, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Fang,Hou, Jian,Chen, Yuan-Yuan,et al. Functional interaction between mutl and 3 '-5 ' exonuclease x in escherichia coli[J]. Archives of biochemistry and biophysics,2010,502(1):39-43. |
| APA | Cheng, Fang.,Hou, Jian.,Chen, Yuan-Yuan.,Zhou, Ying.,Zhang, Hong-Tai.,...&Zhang, Xian-En.(2010).Functional interaction between mutl and 3 '-5 ' exonuclease x in escherichia coli.Archives of biochemistry and biophysics,502(1),39-43. |
| MLA | Cheng, Fang,et al."Functional interaction between mutl and 3 '-5 ' exonuclease x in escherichia coli".Archives of biochemistry and biophysics 502.1(2010):39-43. |
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来源：武汉病毒研究所
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