A high-throughput assay using dengue-1 virus-like particles for drug discovery
文献类型：期刊论文
| 作者 | Qing, Min1,2; Liu, Wei1; Yuan, Zhiming2; Gu, Feng1; Shi, Pei Yong1 |
| 刊名 | Antiviral research
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 86期号:2页码:163-171 |
| 关键词 | Vlp (virus-like particle) Dengue Luciferase reporter Hts (high-throughput screen) |
| ISSN号 | 0166-3542 |
| DOI | 10.1016/j.antiviral.2010.02.313 |
| 通讯作者 | Gu, feng(feng.gu@novartis.com) |
| 英文摘要 | Dengue virus (denv) is a mosquito-borne flavivirus responsible for 50-100 million human infections each year. the development of denv chemotherapy requires high-throughput screening (hts) assays. a dengue virus-like particle (vlp) has been constructed using viral structural proteins to package a renilla luciferase reporter replicon. vlp could be produced by either the sequential electroporation of the replicon rnas and the structural gene rnas or by electroporating replicon rna into a stable cell line expressing the structural proteins. in both approaches, the key to produce high titer vlp (3 x 10(6) foci-forming unit/ml) is to use low temperature (30 degrees c) in the packaging step. in addition, exogenous expression of host protease furin increased vlp infectivity. the infection could be blocked by antibodies against viral envelope protein and by an inhibitor of viral ns5 polymerase, but not by an inhibitor of host alpha-glucosidase (castanospermine). the vlp infection assay was optimized for hts in a 384-well format with consistent and robust signal, providing a simple and rapid cell-based assay for screening inhibitors against denv entry, translation, and replication in an hts format. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | WEST-NILE-VIRUS ; REPLICATION ; ANTIBODY ; FLAVIVIRUSES ; INHIBITOR ; INFECTION ; FUSION ; ORGANIZATION ; MATURATION ; REPLICON |
| WOS研究方向 | Pharmacology & Pharmacy ; Virology |
| WOS类目 | Pharmacology & Pharmacy ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000277741500006 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375776 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gu, Feng |
| 作者单位 | 1.Novartis Inst Trop Dis, Singapore 138670, Singapore 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qing, Min,Liu, Wei,Yuan, Zhiming,et al. A high-throughput assay using dengue-1 virus-like particles for drug discovery[J]. Antiviral research,2010,86(2):163-171. |
| APA | Qing, Min,Liu, Wei,Yuan, Zhiming,Gu, Feng,&Shi, Pei Yong.(2010).A high-throughput assay using dengue-1 virus-like particles for drug discovery.Antiviral research,86(2),163-171. |
| MLA | Qing, Min,et al."A high-throughput assay using dengue-1 virus-like particles for drug discovery".Antiviral research 86.2(2010):163-171. |
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来源：武汉病毒研究所
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