Construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol
文献类型：期刊论文
| 作者 | Liu, Yan; Wei, Qing; Wang, Shu-Jun; Liu, Hong; Zhou, Ning-Yi |
| 刊名 | Applied microbiology and biotechnology
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 87期号:1页码:281-287 |
| 关键词 | Non-antibiotic resistance gene marker Methyl parathion Ortho-nitrophenol Para-nitrophenol Simultaneous degradation |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-010-2457-y |
| 通讯作者 | Liu, hong(liuhong@wh.iov.cn) |
| 英文摘要 | Pseudomonas sp. strain nyz402, a native soil organism that grows on para-nitrophenol (pnp), was genetically engineered for the simultaneous degradation of methyl parathion (mp) and ortho-nitrophenol (onp) by integrating mph (methyl parathion hydrolase gene) from pseudomonas sp. strain wbc-3 and onpab (onp 2-monooxygenase and onp o-benzoquinone reductase genes) from alcaligenes sp. strain nyz215 into the genome of strain nyz402. methyl parathion hydrolase (mph), onp 2-monooxygenase (onpa) and o-benzoquinone reductase (onpb) were constitutively expressed in the engineered strain nyz-mo. strain nyz-mo was free of exogenous antibiotic resistance gene markers and the introduced genes were genetically stable. degradation experiments showed that strain nyz-mo could utilize mp or onp as the sole carbon and energy source, and mineralize 0.1 mm mp-0.1 mm onp simultaneously. this method may serve as a useful strategy for the construction of engineered strains in the degradation of multiple environmental pollutants. |
| WOS关键词 | GRAM-NEGATIVE BACTERIA ; P-NITROPHENOL ; PSEUDOMONAS-AERUGINOSA ; MOLECULAR CHARACTERIZATION ; CLONING ; DEGRADATION ; CATABOLISM ; CLUSTER ; SYSTEM ; ORGANOPHOSPHATE |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000277784100029 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375780 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Liu, Hong |
| 作者单位 | Chinese Acad Sci, State key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yan,Wei, Qing,Wang, Shu-Jun,et al. Construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol[J]. Applied microbiology and biotechnology,2010,87(1):281-287. |
| APA | Liu, Yan,Wei, Qing,Wang, Shu-Jun,Liu, Hong,&Zhou, Ning-Yi.(2010).Construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol.Applied microbiology and biotechnology,87(1),281-287. |
| MLA | Liu, Yan,et al."Construction of an engineered strain free of antibiotic resistance gene markers for simultaneous mineralization of methyl parathion and ortho-nitrophenol".Applied microbiology and biotechnology 87.1(2010):281-287. |
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来源：武汉病毒研究所
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