Biological significance of amino acid substitutions in hepatitis b surface antigen (hbsag) for glycosylation, secretion, antigenicity and immunogenicity of hbsag and hepatitis b virus replication
文献类型：期刊论文
| 作者 | Wu, Chunchen2; Zhang, Xiaoyong1; Tian, Yongjun3; Song, Jianhua2; Yang, Dongliang3; Roggendorf, Michael1; Lu, Mengji2; Chen, Xinwen1,4 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 91页码:483-492 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir.0.012740-0 |
| 通讯作者 | Chen, xinwen(chenxw@pentium.whiov.ac.cn) |
| 英文摘要 | Amino acid substitutions of hepatitis b surface antigen (hbsag) may affect the antigenicity and immunogenicity of hbsag, leading to immune escape and diagnostic failure. the amino acid positions 122 and 160 are known as determinants for hbsag subtypes d/y and w/r, respectively. the substitution k1221 has been shown to strongly affect hbsag antigenicity. in this study, we investigated the significance of naturally occurring amino acid substitutions k1221, t123n, a159g and k160n. both t123n and k160n substitutions resulted in additional n-glycosylated forms of hbsag, while the other mutations produced more glycosylated hbsag compared with the wild type (wt). detection of hbsag by elisa and immunofluorescence staining indicated that variant hbsag (vthbsag) with k1221 was not recognized by hbsag immunoassays, while vthbsag with t123n, all 59g, k160n and a159g/k1son had reduced antigenicity. dna immunization in balb/c mice revealed that wthbsag and vthbsag with t123n and k160n are able to induce antibodies to hbsag (anti-hbs), whereas k1221 and a159g greatly impair the ability of hbsag to trigger anti-hbs responses. the cellular immune response to the hbsag aa 29-38 epitope was enhanced by the k160n substitution. using replication competent clones of hepatitis b virus (hbv), t123n and a159g substitutions were shown to strongly reduce virion assembly. the amino acid substitution k160n appeared to compensate for the negative effect of all 59g on virion production. these results reveal complex effects of amino acid substitutions on biochemical properties of hbsag, on antigenicity and immunogenicity, and on the replication of hbv. |
| WOS关键词 | N-LINKED GLYCOSYLATION ; GENETIC-VARIABILITY ; IMMUNE GLOBULIN ; IN-VITRO ; VARIANTS ; PROTEIN ; ESCAPE ; DETERMINANT ; INFECTION ; ELECTROPORATION |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000274806600019 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375787 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Xinwen |
| 作者单位 | 1.Univ Hosp Essen, Inst Virol, Essen, Germany 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China 3.Huazhong Univ Sci & Technol, Tongji Hosp, Div Clin Immunol, Wuhan 430074, Peoples R China 4.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Microbiol, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Chunchen,Zhang, Xiaoyong,Tian, Yongjun,et al. Biological significance of amino acid substitutions in hepatitis b surface antigen (hbsag) for glycosylation, secretion, antigenicity and immunogenicity of hbsag and hepatitis b virus replication[J]. Journal of general virology,2010,91:483-492. |
| APA | Wu, Chunchen.,Zhang, Xiaoyong.,Tian, Yongjun.,Song, Jianhua.,Yang, Dongliang.,...&Chen, Xinwen.(2010).Biological significance of amino acid substitutions in hepatitis b surface antigen (hbsag) for glycosylation, secretion, antigenicity and immunogenicity of hbsag and hepatitis b virus replication.Journal of general virology,91,483-492. |
| MLA | Wu, Chunchen,et al."Biological significance of amino acid substitutions in hepatitis b surface antigen (hbsag) for glycosylation, secretion, antigenicity and immunogenicity of hbsag and hepatitis b virus replication".Journal of general virology 91(2010):483-492. |
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来源：武汉病毒研究所
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