Proteomic analysis reveals novel binding partners of mip-t3 in human cells
文献类型：期刊论文
| 作者 | Guo, Chao-Wan1; Xiong, Sheng1,2,3; Liu, Ge1; Wang, Yi-Fei2,3; He, Qing-Yu2,3; Zhang, Xian-En4; Zhang, Zhi-Ping4; Ge, Feng1,2,3; Kitazato, Kaio1 |
| 刊名 | Proteomics
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 10期号:12页码:2337-2347 |
| ISSN号 | 1615-9853 |
| 关键词 | Actin Cell biology Hspa8 Interacting proteins Mip-t3 |
| DOI | 10.1002/pmic.201000130 |
| 通讯作者 | Kitazato, kaio(kkholi@nagasaki-u.ac.jp) |
| 英文摘要 | Mip-t3 (microtubule-interacting protein associated with traf3) is a microtubule-interacting protein that evolutionarily conserved from worms to humans, but whose cellular functions remains unknown. to get insight into the functions of mip-t3, we set out to identify mip-t3 interacting proteins by immunoprecipitation in human embryonic kidney 293 cells and ms analysis. as the results, a total of 34 proteins were identified and most of them were novel mip-t3 putative partners. the mip-t3-associated proteins could be grouped into nine clusters based on their molecule functions, including cytoskeleton, chaperone, nucleic acid binding, kinase and so on. three mip-t3-interacted proteins - actin, hspa8 and tubulin were further confirmed by reciprocal coimmunoprecipitations and colocalization analysis. the interaction of mip-t3 with both actin filaments and microtubule suggested that mip-t3 may play an important role in regulation of cytoskeleton dynamics in cells. our results therefore not only uncover a large number of mip-t3-associated proteins that possess a variety of cellular functions, but also provide new research directions for the study of the functions of mip-t3. |
| WOS关键词 | ACTIN CYTOSKELETON ; PROTEIN ; MICROTUBULE ; HSP70 ; GENE ; DISRUPTED-IN-SCHIZOPHRENIA-1 ; COLOCALIZATION ; SCHIZOPHRENIA ; CILIOGENESIS ; BIOGENESIS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000279426300010 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375795 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Kitazato, Kaio |
| 作者单位 | 1.Nagasaki Univ, Grad Sch Biomed Sci, Dept Mol Microbiol & Immunol, Div Mol Pharmacol Infect Agents, Nagasaki 8528521, Japan 2.Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China 3.Jinan Univ, Natl Engn Res Ctr Genet Med, Guangzhou, Guangdong, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Chao-Wan,Xiong, Sheng,Liu, Ge,et al. Proteomic analysis reveals novel binding partners of mip-t3 in human cells[J]. Proteomics,2010,10(12):2337-2347. |
| APA | Guo, Chao-Wan.,Xiong, Sheng.,Liu, Ge.,Wang, Yi-Fei.,He, Qing-Yu.,...&Kitazato, Kaio.(2010).Proteomic analysis reveals novel binding partners of mip-t3 in human cells.Proteomics,10(12),2337-2347. |
| MLA | Guo, Chao-Wan,et al."Proteomic analysis reveals novel binding partners of mip-t3 in human cells".Proteomics 10.12(2010):2337-2347. |
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来源：武汉病毒研究所
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