Polydopamine nanotubes as an effective fluorescent quencher for highly sensitive and selective detection of biomolecules assisted with exonuclease iii amplification
文献类型：期刊论文
| 作者 | Fan, Daoqing1,2; Zhu, Xiaoqing1,2; Zhai, Qingfeng1,2; Wang, Erkang1,2; Dong, Shaojun1,2 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2016-09-20 |
| 卷号 | 88期号:18页码:9158-9165 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b02282 |
| 通讯作者 | Dong, shaojun(dongsj@ciac.ac.cn) |
| 英文摘要 | In this work, the effective fluorescence quenching ability of polydopamine nanotubes (pdants) toward various fluorescent dyes was studied and further applied to fluorescent biosensing for the first time. the pdants could quench the fluorophores with different emission frequencies, aminomethylcoumarin acetate (amca), 6-carboxyfluorescein (fam), 6-carboxytetramethylrhodamine (tamra), and cy5. all the quenching efficiencies reached to more than 97%. taking advantage of pdants' different affinities toward ssdna and dsdna and utilizing the complex of fam-labeled ssdna and pdants as a sensing platform, we achieved highly sensitive and selective detection of human immunodeficiency virus (hiv) dna and adenosine triphosphate (atp) assisted with exonuclease iii amplification. the limits of detection (lods) of hiv dna and atp reached to 3.5 pm and 150 nm, respectively, which were all lower than that of previous nanoquenchers with exo iii amplification, and the platform also presented good applicability in biological samples. fluorescent sensing applications of this nanotube enlightened other targets detection based upon it and enriched the building blocks of fluorescent sensing platforms. this polydopamine nanotube also possesses excellent biocompatibility and biodegradability, which is suitable for future drug delivery, cell imaging, and other biological applications. |
| WOS关键词 | SENSING PLATFORM ; WS2 NANOSHEET ; DNA DETECTION ; IN-VITRO ; NANOSPHERES ; DOPAMINE ; PROBES ; RECOGNITION ; NANOPROBE ; OXIDE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000384038400032 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375805 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dong, Shaojun |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Daoqing,Zhu, Xiaoqing,Zhai, Qingfeng,et al. Polydopamine nanotubes as an effective fluorescent quencher for highly sensitive and selective detection of biomolecules assisted with exonuclease iii amplification[J]. Analytical chemistry,2016,88(18):9158-9165. |
| APA | Fan, Daoqing,Zhu, Xiaoqing,Zhai, Qingfeng,Wang, Erkang,&Dong, Shaojun.(2016).Polydopamine nanotubes as an effective fluorescent quencher for highly sensitive and selective detection of biomolecules assisted with exonuclease iii amplification.Analytical chemistry,88(18),9158-9165. |
| MLA | Fan, Daoqing,et al."Polydopamine nanotubes as an effective fluorescent quencher for highly sensitive and selective detection of biomolecules assisted with exonuclease iii amplification".Analytical chemistry 88.18(2016):9158-9165. |
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来源：中国科学院大学
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