Conjugative transfer of insecticidal plasmid pht73 from bacillus thuringiensis to b. anthracis and compatibility of this plasmid with pxo1 and pxo2
文献类型：期刊论文
| 作者 | Yuan, Yongming; Zheng, Dasheng; Hu, Xiaomin; Cai, Quanxin; Yuan, Zhiming |
| 刊名 | Applied and environmental microbiology
 |
| 出版日期 | 2010-01-15 |
| 卷号 | 76期号:2页码:468-473 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/aem.01984-09 |
| 通讯作者 | Yuan, zhiming(yzm@wh.iov.cn) |
| 英文摘要 | Bacillus anthracis, the etiologic agent of anthrax, is genetically close to and commonly shares a giant gene pool with b. cereus and b. thuringiensis. in view of the human pathogenicity and the long persistence in the environment of b. anthracis, there is growing concern about the effects of genetic exchange with b. anthracis on public health. in this work, we demonstrate that an insecticidal plasmid, pht73, from b. thuringiensis strain kt0 could be efficiently transferred into two attenuated b. anthracis strains, ba63002r (pxo1(+) pxo2(-)) and ba63605r (pxo1(-) pxo2(+)), by conjugation in liquid medium in the laboratory, with transfer rates of 2.3 x 10(-4) and 1.6 x 10(-4) cfu/donor, respectively. the b. anthracis transconjugants containing both pht73 and pxo1 or pxo2 could produce crystal protein cry1ac encoded by plasmid pht73 and had high toxicity to helicoverpa armigera larvae. furthermore, the compatibility and stability of pht73 with pxo1/pxo2 were demonstrated. the data are informative for further investigation of the safety of b. thuringiensis and closely related strains in food and in the environment. |
| WOS关键词 | CEREUS GROUP STRAINS ; SUBSP ISRAELENSIS ; PHENOTYPIC CHARACTERIZATION ; TOXIN GENES ; SEQUENCE ; IDENTIFICATION ; KINETICS ; REPLICON ; SYSTEM ; LARVAE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| 出版者 | AMER SOC MICROBIOLOGY |
| WOS记录号 | WOS:000273354200011 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375806 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Ctr Appl & Environm Microbiol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Yongming,Zheng, Dasheng,Hu, Xiaomin,et al. Conjugative transfer of insecticidal plasmid pht73 from bacillus thuringiensis to b. anthracis and compatibility of this plasmid with pxo1 and pxo2[J]. Applied and environmental microbiology,2010,76(2):468-473. |
| APA | Yuan, Yongming,Zheng, Dasheng,Hu, Xiaomin,Cai, Quanxin,&Yuan, Zhiming.(2010).Conjugative transfer of insecticidal plasmid pht73 from bacillus thuringiensis to b. anthracis and compatibility of this plasmid with pxo1 and pxo2.Applied and environmental microbiology,76(2),468-473. |
| MLA | Yuan, Yongming,et al."Conjugative transfer of insecticidal plasmid pht73 from bacillus thuringiensis to b. anthracis and compatibility of this plasmid with pxo1 and pxo2".Applied and environmental microbiology 76.2(2010):468-473. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。