The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through tnf pathway in oyster crassostrea gigas
文献类型：期刊论文
| 作者 | Liu, Zhaoqun1,3; Zhou, Zhi1; Wang, Lingling2; Dong, Wenjing1; Qiu, Limei1; Song, Linsheng2 |
| 刊名 | Developmental and comparative immunology
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 65页码:139-148 |
| 关键词 | Crassostrea gigas Acetylcholine Muscarinic acetylcholine receptor Tnf pathway Immune regulation |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2016.07.003 |
| 通讯作者 | Wang, lingling(wanglingling@ms.qdio.ac.cn) ; Song, linsheng(lshsong@dlou.edu.cn) |
| 英文摘要 | Muscarinic receptors, which selectively take muscarine as their ligand, are critical for the immunological and physiological processes in animals. in the present study, the open region frame (orf) of a homologue of muscarinic acetylcholine (ach) receptor (machr) was amplified from oyster crassostrea gigas (named as cgmachr-1), whose full length was 1983 bp and the protein it encoded contained 660 amino acids with a seven transmembrane region. phylogeny analysis suggested that cgmachr-1 shared homology with m-5 muscarinic receptor found in invertebrates including habropoda laboriosa, acromyrmex echinatior and echinococcus granulosus. after cell transfection of cgrnachr-1 into hek293t cells and ach incubation, the level of intracellular ca2+ and camp increased significantly (p < 0.05). such trend could be reverted with the addition of m-3 and m-5 muscarinic receptor antagonists damp and dar. the cgmachr-1 transcripts were ubiquitously detectable in seven different tissues with the maximal expression level in adductor muscle. when the oysters received lps stimulation, cgmachr-1 mrna expression in haemocyte was increased to the highest level (6.05-fold, p < 0.05) at 24 h, while blocking cgmachr-1 using receptor antagonists before lps stimulation promoted the expression of oyster tnf, resulting in the increase of haemocyte apoptosis index. these results suggested that cgmachr-1 was the key molecule in cholinergic neuroendocrine-immune system contributing to the regulation of tnf expression and apoptosis process. (c) 2016 elsevier ltd. all rights reserved. |
| WOS关键词 | SCALLOP CHLAMYS-FARRERI ; VIBRIO-ANGUILLARUM CHALLENGE ; ANTIINFLAMMATORY PATHWAY ; NERVOUS-SYSTEM ; ROS GENERATION ; T-LYMPHOCYTE ; IMMUNOMODULATION ; LOCALIZATION ; INFLAMMATION ; MODULATION |
| WOS研究方向 | Immunology ; Zoology |
| WOS类目 | Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000384388400016 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375807 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Lingling; Song, Linsheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,et al. The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through tnf pathway in oyster crassostrea gigas[J]. Developmental and comparative immunology,2016,65:139-148. |
| APA | Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,Dong, Wenjing,Qiu, Limei,&Song, Linsheng.(2016).The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through tnf pathway in oyster crassostrea gigas.Developmental and comparative immunology,65,139-148. |
| MLA | Liu, Zhaoqun,et al."The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through tnf pathway in oyster crassostrea gigas".Developmental and comparative immunology 65(2016):139-148. |
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来源：中国科学院大学
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