Characterization of the nuclear import and export mechanisms of bovine herpesvirus-1 infected cell protein 27
文献类型：期刊论文
| 作者 | Ding, Qiong1; Guo, Hong1; Lin, Fusen1; Pan, Weiwei1; Ye, Bin2; Zheng, Alan C.1 |
| 刊名 | Virus research
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 149期号:1页码:95-103 |
| 关键词 | Bovine herpesvirus-1 infected cell protein 27 Nuclear import Nuclear export Ran-gtp Chromosomal region maintenance 1 (crm1) |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2010.01.009 |
| 通讯作者 | Zheng, alan c.(zheng.alan@hotmail.com) |
| 英文摘要 | In previous study, we have identified a nuclear localization signal (nls) and a nucleolar localization signal (nols) in bovine herpesvirus-1 (bhv-1) infected cell protein 27 (bicp27), which targets predominantly to the nucleolus. furthermore, the c-terminal 300 amino acid residues targets exclusively to the cytoplasm, suggesting that bicp27 might contain a nuclear export signal (nes). amino acid sequence analysis revealed that there is a cluster of leucine-rich residues resembling a nes. heterokaryon assays demonstrated that bicp27 is capable of shuttling between the nucleus and the cytoplasm of the bhv-1 infected, bicp27 and bicp27-eyfp transfected cells. deletion mutant analysis revealed that this property is attributed to the leucine-rich nes 299leelcaarrlsl310. moreover, the functional nes could mediate transport of a monomer eyfp and a dimer eyfp to the cytoplasm. the nucleocytoplasmic shuttling of bicp27 and the nuclear export of nes-eyfp and nes-deyfp could be blocked by leptomycin lmb, an inhibitor of the chromosomal region maintenance 1 (crm1), which is the receptor for exportin-1-dependent nuclear export. in addition, the nuclear import of bicp27 was inhibited by a dominant negative ran-gtp, namely ran-gtp q69l, indicating that bicp27 localized to the nucleus by means of a classic ran dependent nuclear import mechanism. in conclusion, these results demonstrate that bicp27 shuttles between the nucleus and the cytoplasm by the functional nes and nls through a crm1-dependent nuclear export pathway and a ran dependent nuclear import pathway. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | SIMPLEX-VIRUS TYPE-1 ; REGULATORY PROTEIN ; MESSENGER-RNA ; NUCLEOCYTOPLASMIC TRANSPORT ; TRANSACTIVATOR PROTEIN ; HNRNP PROTEINS ; PORE COMPLEX ; CYTOPLASM ; SIGNAL ; SHUTTLES |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000276341900013 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375813 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Alan C. |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Mol Virol & Viral Immunol Res Grp, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chongqing Med Univ, Dept Pathogenbiol, Chongqing 400016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Qiong,Guo, Hong,Lin, Fusen,et al. Characterization of the nuclear import and export mechanisms of bovine herpesvirus-1 infected cell protein 27[J]. Virus research,2010,149(1):95-103. |
| APA | Ding, Qiong,Guo, Hong,Lin, Fusen,Pan, Weiwei,Ye, Bin,&Zheng, Alan C..(2010).Characterization of the nuclear import and export mechanisms of bovine herpesvirus-1 infected cell protein 27.Virus research,149(1),95-103. |
| MLA | Ding, Qiong,et al."Characterization of the nuclear import and export mechanisms of bovine herpesvirus-1 infected cell protein 27".Virus research 149.1(2010):95-103. |
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来源：武汉病毒研究所
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