Recombinant flagellins with partial deletions of the hypervariable domain lose antigenicity but not mucosal adjuvancy
文献类型：期刊论文
| 作者 | Liu, Fang1; Yang, Jingyi1; Zhang, Yan1,2,3; Zhou, Dihan1,2,3; Chen, Yaoqing2,3; Gai, Weiwei1,2,3; Shi, Wei1; Li, Qiaoli1; Tien, Po2,3; Yan, Huimin1,2,3 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2010-02-19 |
| 卷号 | 392期号:4页码:582-587 |
| 关键词 | Flagellin Antigenicity Immunogenicity Cytokine Mucosal immunoresponse |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2010.01.077 |
| 通讯作者 | Yan, huimin(hmyan@wh.iov.cn) |
| 英文摘要 | Flagellin contains conserved n/c domains for tlr5 binding to activate innate immunity and a middle hypervariable domain harboring the major antigenic epitopes. however, conflict results existed in the previous studies as to whether the hypervariable domain was involved in the cytokine production and adjuvancy of flagellin. here we constructed three flagellin variants (designated as flic delta 190-278, flic delta 220-320, and flic delta 180-400) with deletions in the hypervariable domain. our data demonstrated that all deletion variants lost substantial antigenicity but not mucosal adjuvancy. surprisingly, the variant with deletion of amino acids 220-320 (flic delta 220-320) induced higher production of il-8, mcp-1, and tnf-alpha, and showed higher mucosal adjuvancy than full-length flic flagellin. our data supported the notion that the hypervariable domain was involved in the cytokine production by flagellin and more importantly demonstrated that the hypervariable domain was important for the mucosal adjuvancy of flagellin. (c) 2010 elsevier inc. all rights reserved. |
| WOS关键词 | TOLL-LIKE RECEPTOR-5 ; ESCHERICHIA-COLI ; BACTERIAL FLAGELLIN ; ACTIVATION ; IMMUNITY ; REGION ; RESPONSES ; MOTILITY ; PROTEIN ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000275896900022 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375815 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yan, Huimin |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Fang,Yang, Jingyi,Zhang, Yan,et al. Recombinant flagellins with partial deletions of the hypervariable domain lose antigenicity but not mucosal adjuvancy[J]. Biochemical and biophysical research communications,2010,392(4):582-587. |
| APA | Liu, Fang.,Yang, Jingyi.,Zhang, Yan.,Zhou, Dihan.,Chen, Yaoqing.,...&Yan, Huimin.(2010).Recombinant flagellins with partial deletions of the hypervariable domain lose antigenicity but not mucosal adjuvancy.Biochemical and biophysical research communications,392(4),582-587. |
| MLA | Liu, Fang,et al."Recombinant flagellins with partial deletions of the hypervariable domain lose antigenicity but not mucosal adjuvancy".Biochemical and biophysical research communications 392.4(2010):582-587. |
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来源：武汉病毒研究所
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