Recombinant n-terminal part of bovine herpesviru 1 icp27 protein: its preparation, purification, and use for raising specific antiserum
文献类型：期刊论文
| 作者 | Zhao, L.1; Ren, X.2; Guo, H.1; Ding, Q.1; Zheng, A. C.1 |
| 刊名 | Acta virologica
 |
| 出版日期 | 2010 |
| 卷号 | 54期号:2页码:147-150 |
| 关键词 | Bovine herpesvirus 1 Icp27 Recombinant protein Antiserum Immunofluorescence assay |
| ISSN号 | 0001-723X |
| DOI | 10.4149/av_2010_02_147 |
| 通讯作者 | Zheng, a. c.(zheng.alan@hotmail.com) |
| 英文摘要 | Recombinant n-terminal part of bovine herpesvirus-1 (bohv-1) 1cp27 protein fused with thioredoxin and his-tag ("the recombinant protein") expressed in escherichia coli was purified by the ni(2+)-nta affinity chromatography and used for the preparation of antiserum by immunization of rabbits. the antiserum recognized the recombinant protein in western blot analysis and was able to detect bohv-1 icp27 in the nucleoli of bohv-1-infected mdbk cells. these results showed that such an antiserum could serve as a valuable tool in further studies of the functions of bohv-1 icp27. |
| WOS关键词 | VIRUS TYPE-1 ICP27 ; REGULATORY PROTEIN ; BICP27 ; GENE ; EXPRESSION ; DNA |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000279588300010 |
| 出版者 | AEPRESS SRO |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375825 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, A. C. |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.China Agr Univ, Dept Anim Sci, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, L.,Ren, X.,Guo, H.,et al. Recombinant n-terminal part of bovine herpesviru 1 icp27 protein: its preparation, purification, and use for raising specific antiserum[J]. Acta virologica,2010,54(2):147-150. |
| APA | Zhao, L.,Ren, X.,Guo, H.,Ding, Q.,&Zheng, A. C..(2010).Recombinant n-terminal part of bovine herpesviru 1 icp27 protein: its preparation, purification, and use for raising specific antiserum.Acta virologica,54(2),147-150. |
| MLA | Zhao, L.,et al."Recombinant n-terminal part of bovine herpesviru 1 icp27 protein: its preparation, purification, and use for raising specific antiserum".Acta virologica 54.2(2010):147-150. |
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来源：武汉病毒研究所
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