Real-time visualization of prion transport in single live cells using quantum dots
文献类型：期刊论文
| 作者 | Luo, Kan2,3; Li, Shu4; Xie, Min1; Wu, Di2,3; Wang, WenXi2,3; Chen, Rui2,3; Huang, Liqin2,3; Huang, Tao2,3; Pang, Daiwen1; Xiao, Gengfu2,3,5 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2010-04-09 |
| 卷号 | 394期号:3页码:493-497 |
| 关键词 | Prion Quantum dot Visualization Live cell Lipid raft |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2010.02.159 |
| 通讯作者 | Pang, daiwen() |
| 英文摘要 | Prion diseases ale fatal neurodegenerative disorders resulting from structural conversion of the cellular isoform of prp(c) to the infectious scrapie isoform prp(sc). it is believed that such structural alteration may occur within the internalization pathway however, there is no direct evidence to support this hypothesis employing quantum dots (qds) as a probe, we have recorded a real-time movie demonstrating the process of prion internalization in a living cell for the first time the entire internalization process can be divided into four discrete but connected stages in addition, using methyl-beta-cyclodextrin to disrupt cell membrane cholesterol, we show that lipid rafts play an important role in locating cellular prp(c) to the cell membrane and in initiating prp(c) endocytosis (c) 2010 elsevier inc all rights reserved |
| WOS关键词 | LIPID RAFTS ; PROTEIN ; TRACKING ; INFECTION ; BIOLOGY ; BINDING ; PRPC |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000276785900011 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375828 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Pang, Daiwen |
| 作者单位 | 1.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China 2.Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Modern Virol Res Ctr, Coll Life Sci, Wuhan 430072, Peoples R China 4.Chinese Acad Med Sci, Inst Pathogen Biol, AIDS Res Ctr, Beijing 100730, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Kan,Li, Shu,Xie, Min,et al. Real-time visualization of prion transport in single live cells using quantum dots[J]. Biochemical and biophysical research communications,2010,394(3):493-497. |
| APA | Luo, Kan.,Li, Shu.,Xie, Min.,Wu, Di.,Wang, WenXi.,...&Xiao, Gengfu.(2010).Real-time visualization of prion transport in single live cells using quantum dots.Biochemical and biophysical research communications,394(3),493-497. |
| MLA | Luo, Kan,et al."Real-time visualization of prion transport in single live cells using quantum dots".Biochemical and biophysical research communications 394.3(2010):493-497. |
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来源：武汉病毒研究所
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