Copper (ii) promotes the formation of soluble neurotoxic prp oligomers in acidic environment
文献类型：期刊论文
| 作者 | Wu, Di2,3; Zhang, Wentao2,3; Luo, Qingying4; Luo, Kan2,3; Huang, Liqin2,3; Wang, Wenxi2,3; Huang, Tao2,3; Chen, Rui2,3; Lin, Yi4; Pang, Daiwen4 |
| 刊名 | Journal of cellular biochemistry
 |
| 出版日期 | 2010-10-15 |
| 卷号 | 111期号:3页码:627-633 |
| 关键词 | Prion Oligomer Copper Acidic ph Toxicity |
| ISSN号 | 0730-2312 |
| DOI | 10.1002/jcb.22743 |
| 通讯作者 | Xiao, gengfu(xiaogf@wh.iov.cn) |
| 英文摘要 | Prion diseases are classically considered to be "amyloid diseases" caused by the deposition of amyloid fibrils in the brain. recent studies identified soluble oligomers of prp (prion protein) in damaged neuronal tissue. however, the details of prp oligomerization are still unclear. in this study, we demonstrate that cu(2+) plays a vital role in the formation of soluble prp oligomers. a cu(2+)-triggered structural conversion of prp (90-231) to a beta-sheet isoform in ph 5.0 buffer was revealed by circular dichroism spectra and fluorescence measurement. soluble oligomers were isolated by size exclusion chromatography from experimental solutions, allowing atomic force microscopy to reveal their morphology. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) and flow cytometry assays demonstrated that oligomeric prp induced significant damage in and apoptosis of neuroblastoma sk-n-sh cells. these results indicate that in an acidic environment, cu(2+) promotes the formation of neurotoxic soluble prp oligomers. j. cell. biochem. 111: 627-633, 2010. (c) 2010 wiley-liss, inc. |
| WOS关键词 | HUMAN PRION PROTEIN ; SCRAPIE ISOFORM ; COMMON MECHANISM ; BINDING-SITES ; PEPTIDES ; DISEASE ; REGION ; CU2+ ; NEURODEGENERATION ; COORDINATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000282559200012 |
| 出版者 | WILEY-LISS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375830 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xiao, Gengfu |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China 4.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Di,Zhang, Wentao,Luo, Qingying,et al. Copper (ii) promotes the formation of soluble neurotoxic prp oligomers in acidic environment[J]. Journal of cellular biochemistry,2010,111(3):627-633. |
| APA | Wu, Di.,Zhang, Wentao.,Luo, Qingying.,Luo, Kan.,Huang, Liqin.,...&Xiao, Gengfu.(2010).Copper (ii) promotes the formation of soluble neurotoxic prp oligomers in acidic environment.Journal of cellular biochemistry,111(3),627-633. |
| MLA | Wu, Di,et al."Copper (ii) promotes the formation of soluble neurotoxic prp oligomers in acidic environment".Journal of cellular biochemistry 111.3(2010):627-633. |
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来源：武汉病毒研究所
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