A new method for the detection of the h5 influenza virus by magnetic beads capturing quantum dot fluorescent signals
文献类型：期刊论文
| 作者 | Zhang, Wentao2,3; Wu, Di2,3; Wei, Jianghua2,3; Xiao, Gengfu1,2,3 |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 32期号:12页码:1933-1937 |
| 关键词 | Hybridization Influenza virus Magnetic beads Quantum dots |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-010-0379-5 |
| 通讯作者 | Xiao, gengfu(xiaogf@wh.iov.cn) |
| 英文摘要 | A new method to detect h5 influenza virus using quantum dots (qds) and magnetic beads (mbs) is described. qds conjugated with oligonucleotide probes were used to produce fluorescent signals and mbs, that were also conjugated with probes, were used to isolate and concentrate the signals. target viral rnas led to a sandwich hybridization between the functionalized qds and mbs. one-step hybridization facilitated the subtype determination. as little as 0.1 ng viral rna could be detected. |
| WOS关键词 | DNA ; PCR |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000285912900025 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375835 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xiao, Gengfu |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wentao,Wu, Di,Wei, Jianghua,et al. A new method for the detection of the h5 influenza virus by magnetic beads capturing quantum dot fluorescent signals[J]. Biotechnology letters,2010,32(12):1933-1937. |
| APA | Zhang, Wentao,Wu, Di,Wei, Jianghua,&Xiao, Gengfu.(2010).A new method for the detection of the h5 influenza virus by magnetic beads capturing quantum dot fluorescent signals.Biotechnology letters,32(12),1933-1937. |
| MLA | Zhang, Wentao,et al."A new method for the detection of the h5 influenza virus by magnetic beads capturing quantum dot fluorescent signals".Biotechnology letters 32.12(2010):1933-1937. |
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来源：武汉病毒研究所
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