Peg-interspersed nitrilotriacetic acid-functionalized quantum dots for site-specific labeling of prion proteins expressed on cell surfaces
文献类型：期刊论文
| 作者 | Xie, Min3; Luo, Kan1,2; Huang, Bi-Hai3; Liu, Shu-Lin3; Hu, Jun3; Cui, Di3; Zhang, Zhi-Ling3; Xiao, Geng-Fu1,2,4; Pang, Dai-Wen3 |
| 刊名 | Biomaterials
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 31期号:32页码:8362-8370 |
| 关键词 | Nitrilotriacetic acid Quantum dots Prion Site-specific Nanobioprobe |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2010.07.063 |
| 通讯作者 | Xiao, geng-fu(xiaogf@wh.iov.cn) |
| 英文摘要 | A strategy has been put forward to fabricate peg-interspersed nitrilotriacetic acid (nta)-functionalized qds by one-step self-assembly using a mixture of self-synthesized nta-terminated amphiphilic polymer and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-n-[carboxy(polyethylene glycol)2000] (dspe-peg-cooh). the process was highly reproducible for facile functionalization of qds via simultaneous self-assembly of biocompatible peg molecules onto their surface. an optimized molar ratio of nta-terminated amphiphilic polymer to dspe peg cooh was used to obtain nta-functionalized qds for site-specific labeling of prion proteins (prp(c)) expressed on cell surfaces. fabricated nta-functionalized qds can be a good candidate used for real-time visualization of prp(c) in single live cells so as to clarify the nosogenesis of pathogenic scrapie prion protein (prp(sc)). (c) 2010 elsevier ltd. all rights reserved. |
| WOS关键词 | HISTIDINE-TAGGED PROTEINS ; LIVE CELLS ; PHOSPHOLIPID MICELLES ; NANOCRYSTALS ; SINGLE ; RECOMBINANT ; PURIFICATION ; BINDING ; PROBES ; CDSE |
| WOS研究方向 | Engineering ; Materials Science |
| WOS类目 | Engineering, Biomedical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| WOS记录号 | WOS:000283112700027 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375840 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xiao, Geng-Fu |
| 作者单位 | 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Min,Luo, Kan,Huang, Bi-Hai,et al. Peg-interspersed nitrilotriacetic acid-functionalized quantum dots for site-specific labeling of prion proteins expressed on cell surfaces[J]. Biomaterials,2010,31(32):8362-8370. |
| APA | Xie, Min.,Luo, Kan.,Huang, Bi-Hai.,Liu, Shu-Lin.,Hu, Jun.,...&Pang, Dai-Wen.(2010).Peg-interspersed nitrilotriacetic acid-functionalized quantum dots for site-specific labeling of prion proteins expressed on cell surfaces.Biomaterials,31(32),8362-8370. |
| MLA | Xie, Min,et al."Peg-interspersed nitrilotriacetic acid-functionalized quantum dots for site-specific labeling of prion proteins expressed on cell surfaces".Biomaterials 31.32(2010):8362-8370. |
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来源：武汉病毒研究所
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