Role of suppressor of cytokine signaling 3 in lipid metabolism: analysis based on a phage-display human liver cdna library
文献类型：期刊论文
| 作者 | Luo, Bin1; Zou, Tingting3,4; Lu, Nan1; Chai, Fan2; Ye, Xing1; Wang, YeFu1; Qi, Yipeng1 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2011-12-09 |
| 卷号 | 416期号:1-2页码:39-44 |
| 关键词 | Obesity Suppressor of cytokine signaling 3 Very-long-chain acyl-coa dehydrogenase Leptin-resistance Fatty acid beta-oxidation |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2011.10.129 |
| 通讯作者 | Wang, yefu(wangyefu@whu.edu.cn) |
| 英文摘要 | Suppressor of cytokine signaling 3 (socs3) is a likely mediator of feedback inhibition on the leptin receptor and may cause physiological leptin-resistance, leading to the development of obesity. the aim of this study was to identify potential peptides interacting with purified socs3 by using a phage-display human liver cdna library. we developed a t7 select phage-display system with purified socs3 as bait to screen a human liver cdna library. after 4 rounds of screening and sequencing analysis, we found that phage-presenting peptide rggvvtsnplgf show significant binding to socs3. the peptide sequence was similar to the sequence of amino acids 644-655 of c-terminal extra-polypeptide of very-long-chain acyl-coa dehydrogenase (vlcad), which is 1 of 4 flavoproteins that catalyzing the initial step of the mitochondrial fatty acid beta-oxidation, implying a close relationship between socs3 and vlcad. we identified vlcad as a novel socs3 interacting protein both in vitro and vivo, and found that socs3 mediates the ubiquitination pathway for proteasomal degradation of vlcad c-terminal extra-polypeptide via its socs-box. animal experimentation demonstrated that vlcad is functionally involved in socs3 binding and thus. socs3 play an important role in the regulation of fatty acid p-oxidation. in conclusion, socs3 is an important factor for lipid metabolism and a potential drug-target for treatment of widespread obesity. (c) 2011 published by elsevier inc. |
| WOS关键词 | COENZYME-A DEHYDROGENASE ; SOCS-BOX MOTIF ; LEPTIN RESISTANCE ; OBESE GENE ; OXIDATION ; PURIFICATION ; DEGRADATION ; PROTEIN ; SYSTEM ; RISK |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000298222100007 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375868 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, YeFu |
| 作者单位 | 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Med Sci, Inst Pathogen Biol, State Key Lab Mol Virol & Genet Engn, Beijing 100176, Peoples R China 4.Peking Union Med Coll, Beijing 100176, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Bin,Zou, Tingting,Lu, Nan,et al. Role of suppressor of cytokine signaling 3 in lipid metabolism: analysis based on a phage-display human liver cdna library[J]. Biochemical and biophysical research communications,2011,416(1-2):39-44. |
| APA | Luo, Bin.,Zou, Tingting.,Lu, Nan.,Chai, Fan.,Ye, Xing.,...&Qi, Yipeng.(2011).Role of suppressor of cytokine signaling 3 in lipid metabolism: analysis based on a phage-display human liver cdna library.Biochemical and biophysical research communications,416(1-2),39-44. |
| MLA | Luo, Bin,et al."Role of suppressor of cytokine signaling 3 in lipid metabolism: analysis based on a phage-display human liver cdna library".Biochemical and biophysical research communications 416.1-2(2011):39-44. |
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来源：武汉病毒研究所
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