Screening and identification of host factors interacting with ul14 of herpes simplex virus 1
文献类型：期刊论文
| 作者 | Wu, Fuqing; Xing, Junji; Wang, Shuai; Li, Meili; Zheng, Chunfu |
| 刊名 | Medical microbiology and immunology
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 200期号:3页码:203-208 |
| 关键词 | Herpes simplex virus 1 Ul14 Yeast two-hybrid Co-localization Co-immunoprecipitation |
| ISSN号 | 0300-8584 |
| DOI | 10.1007/s00430-011-0196-z |
| 通讯作者 | Zheng, chunfu(zheng.alan@hotmail.com) |
| 英文摘要 | The ul14 protein of herpes simplex virus type 1 (hsv-1) is highly conserved in herpesvirus family. however, its exact function during the hsv-1 replication cycle is little known. in the present study, a high throughput yeast two-hybrid system was employed to screen the cellular factors interacting with ul14, and five target candidates were yielded: (1) tsc22 domain family protein 3 (tsc22d3); (2) mediator of rna polymerase ii transcription subunit 8 isoform 1(med8); (3) runt-related transcription factor 3 (runx3); (4) arrestin beta-2 (arrb2); (5) cereblon (crbn). indirect immunofluorescent assay showed that both tsc22d3 and med8 co-localized with ul14. co-immunoprecipitation assay demonstrated that ul14 could be immunoprecipitated by tsc22d3, suggesting that ul14 interacted with tsc22d3 under physiological condition. in summary, this study opened up new avenues toward delineating the function and physiological significance of ul14 during the hsv-1 replication cycle. |
| WOS关键词 | PROTECTS T-LYMPHOCYTES ; GENE-PRODUCT ; MEDIATOR ; ACTIVATION ; APOPTOSIS ; TYPE-1 ; GILZ ; BETA-ARRESTIN-2 ; MECHANISM ; TARGET |
| WOS研究方向 | Immunology ; Microbiology |
| WOS类目 | Immunology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000292833900007 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375874 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Chunfu |
| 作者单位 | Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Fuqing,Xing, Junji,Wang, Shuai,et al. Screening and identification of host factors interacting with ul14 of herpes simplex virus 1[J]. Medical microbiology and immunology,2011,200(3):203-208. |
| APA | Wu, Fuqing,Xing, Junji,Wang, Shuai,Li, Meili,&Zheng, Chunfu.(2011).Screening and identification of host factors interacting with ul14 of herpes simplex virus 1.Medical microbiology and immunology,200(3),203-208. |
| MLA | Wu, Fuqing,et al."Screening and identification of host factors interacting with ul14 of herpes simplex virus 1".Medical microbiology and immunology 200.3(2011):203-208. |
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来源：武汉病毒研究所
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