Production and characterization of monoclonal antibodies against the hemagglutinin of h5n1 and antigenic investigation of avian influenza h5n1 viruses isolated from china
文献类型：期刊论文
| 作者 | Wei, Jie; Yan, Bing; Chen, Ze; Li, Tianxian; Deng, Fei; Wang, Hualin; Hu, Zhihong |
| 刊名 | Canadian journal of microbiology
 |
| 出版日期 | 2011 |
| 卷号 | 57期号:1页码:42-48 |
| 关键词 | Antigenic characterization Epitope H5n1 Monoclonal antibodies Phylogeny |
| ISSN号 | 0008-4166 |
| DOI | 10.1139/w10-097 |
| 通讯作者 | Hu, zhihong(huzh@wh.iov.cn) |
| 英文摘要 | Eight monoclonal antibodies against hemagglutinin of influenza a virus a/chicken/henan/01/2004(h5n1) were produced by a dna prime and inactivated virions-boost immunization strategy. among the monoclonal antibodies, 3 (h50, h56, and h57) exhibited hemagglutination inhibition activity. western blot analyses revealed that all the monoclonal antibodies reacted to the prokaryotically expressed ha1 of a/chicken/henan/01/2004(h5n1). the monoclonal antibodies were then used to characterize 10 avian influenza h5n1 viruses isolated from china during 2004 to 2007, by using the hemagglutination inhibition test and the antigen-capture enzyme-linked immunosorbent assay. the isolates could be divided into 4 different antigenic groups according to their responses to the monoclonal antibodies. the antigenic grouping of these 10 h5n1 isolates, using these antibodies, did not completely match their phylogenetic classification based on the hemagglutinin sequences. the results showed there were antigenic variations within the subclade 2.3.4 of h5n1, which is predominant in china. |
| WOS关键词 | HA DNA VACCINE ; A VIRUS ; IMMUNIZATION ; PROTEIN ; PROTECTION ; HUMANS ; BIRDS ; ASIA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000286474800005 |
| 出版者 | CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375875 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, Zhihong |
| 作者单位 | Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Jie,Yan, Bing,Chen, Ze,et al. Production and characterization of monoclonal antibodies against the hemagglutinin of h5n1 and antigenic investigation of avian influenza h5n1 viruses isolated from china[J]. Canadian journal of microbiology,2011,57(1):42-48. |
| APA | Wei, Jie.,Yan, Bing.,Chen, Ze.,Li, Tianxian.,Deng, Fei.,...&Hu, Zhihong.(2011).Production and characterization of monoclonal antibodies against the hemagglutinin of h5n1 and antigenic investigation of avian influenza h5n1 viruses isolated from china.Canadian journal of microbiology,57(1),42-48. |
| MLA | Wei, Jie,et al."Production and characterization of monoclonal antibodies against the hemagglutinin of h5n1 and antigenic investigation of avian influenza h5n1 viruses isolated from china".Canadian journal of microbiology 57.1(2011):42-48. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。