Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus orf51 is a chab homologous gene involved in budded virus production and dna replication
文献类型：期刊论文
| 作者 | Zheng, Fangliang1,2; Huang, Yi1; Long, Gang1; Sun, Xiulian1; Wang, Hanzhong1 |
| 刊名 | Virus research
 |
| 出版日期 | 2011 |
| 卷号 | 155期号:1页码:203-212 |
| 关键词 | Baculovirus Hearnpv orf51 (ha51) Chab Bv production Dna replication |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2010.10.006 |
| 通讯作者 | Huang, yi(hy@wh.iov.cn) |
| 英文摘要 | The baculovirus chab proteins are conserved in all completely sequenced lepidopteran npvs and are annotated as putative dna binding proteins. here we investigated helicoverpa annigera single nucleocapsid nucleopolyhedrovirus (hearnpv) orf51 (ha51). one of the chab homologues in hearnpv. 5`-race revealed that ha51 is transcribed from a conventional early promoter transcriptional initiator motif (catt) located at 159nt upstream of atg. rt-pcr confirmed that ha51 is an early transcribed gene. to study the function of ha51 in the life cycle of hearnpv, ha51 knockout and repair bacmids were generated by homologous recombination in escherichia coli. growth curve and dna replication analyses showed that the levels of budded virus (bv) production and viral dna accumulation were significantly higher in cells infected with ha51 null virus than those infected with wild-type bacmid derived virus. electron microscopy revealed that polyhedra formation was not affected by the deletion of ha51. bioassay demonstrated that the ha51-deleted virus had similar oral infectivity as the wild-type and rescued virus. western blot analyses suggested that ha51 is a component of the nucleocapsid of bv and occlusion-derived virus as well as the envelope of bv. immunofluorescence microscopy showed that ha51 protein is mainly localized in the cytoplasm of infected cells. taken together, our results indicate that, unlike previously characterized baculovirual chab genes, ha51 is involved in viral dna replication and bv production and is transcribed in the early stage of infection. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | CELL-CYCLE ARREST ; AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS ; ESCHERICHIA-COLI ; MULTICAPSID NUCLEOPOLYHEDROVIRUS ; ACCUMULATION ; PROTEINS ; SEQUENCE ; GENOMES ; CLONING ; PHASE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000287010300028 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375881 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Huang, Yi |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Fangliang,Huang, Yi,Long, Gang,et al. Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus orf51 is a chab homologous gene involved in budded virus production and dna replication[J]. Virus research,2011,155(1):203-212. |
| APA | Zheng, Fangliang,Huang, Yi,Long, Gang,Sun, Xiulian,&Wang, Hanzhong.(2011).Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus orf51 is a chab homologous gene involved in budded virus production and dna replication.Virus research,155(1),203-212. |
| MLA | Zheng, Fangliang,et al."Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus orf51 is a chab homologous gene involved in budded virus production and dna replication".Virus research 155.1(2011):203-212. |
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来源：武汉病毒研究所
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