Characterization of ssu5c promoter of a rbcs gene from duckweed (lemna gibba)
文献类型：期刊论文
| 作者 | Wang, Youru1,2,3; Zhang, Yong1; Yang, Baoyu1; Chen, Shiyun1 |
| 刊名 | Molecular biology reports
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 38期号:4页码:2563-2568 |
| 关键词 | Duckweed Lemna gibba Rbcs Ssu5c promoter Tissue-specific expression Phytochrome |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-010-0395-5 |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) |
| 英文摘要 | Photosynthesis-associated nuclear genes are able to respond to multiple environmental and developmental signals. studies have shown that light signals coordinate with hormone signaling pathways to control photomorphogenesis. a small subunit of ribulose-1,5 bisphosphate carboxylase/oxygenase (rbcs) gene promoter was cloned from duckweed (lemna gibba). sequence analysis revealed this promoter is different from the previously reported rbcs promoters and is named ssu5c. analysis of t-1 transgenic tobacco plants with a reporter gene under the control of the ssu5c promoter revealed that this promoter is tissue-specific and is positively regulated by red light. promoter deletion analysis confirmed a region from position -152 to -49 relative to the start of transcription containing boxes x, y and z, and is identified to be critical for phytochrome responses. further functional analysis of constructs of box-x, y, z, which was respectively fused to the basal ssu5c promoter, defined boxes x, y and z alone are able to direct phytochrome-regulated expression, indicating that boxes y and z are different from those of the ssu5b promoters in l. gibba. this promoter may be used for plant gene expression in a tissue-specific manner. |
| WOS关键词 | LIGHT-RESPONSIVE UNIT ; FUNCTIONAL-PROPERTIES ; PLANT PROMOTERS ; SMALL-SUBUNIT ; PHYTOCHROME ; EXPRESSION ; CARBOXYLASE ; CULTURES ; BINDING ; GROWTH |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000289257100043 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375886 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Hubei Normal Univ, Life Sci Coll, Huangshi 435002, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Youru,Zhang, Yong,Yang, Baoyu,et al. Characterization of ssu5c promoter of a rbcs gene from duckweed (lemna gibba)[J]. Molecular biology reports,2011,38(4):2563-2568. |
| APA | Wang, Youru,Zhang, Yong,Yang, Baoyu,&Chen, Shiyun.(2011).Characterization of ssu5c promoter of a rbcs gene from duckweed (lemna gibba).Molecular biology reports,38(4),2563-2568. |
| MLA | Wang, Youru,et al."Characterization of ssu5c promoter of a rbcs gene from duckweed (lemna gibba)".Molecular biology reports 38.4(2011):2563-2568. |
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来源：武汉病毒研究所
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