Ampk regulates autophagy by phosphorylating becn1 at threonine 388
文献类型：期刊论文
| 作者 | Zhang, Deyi1,2; Wang, Wei1,2; Sun, Xiujie1,2; Xu, Daqian1,2; Wang, Chenyao1,2; Zhang, Qian1,2; Wang, Huafei1,2; Luo, Wenwen1,2; Chen, Yan1,2; Chen, Huaiyong3 |
| 刊名 | Autophagy
 |
| 出版日期 | 2016 |
| 卷号 | 12期号:9页码:1447-1459 |
| 关键词 | Ampk Autophagy Becn1 Phosphorylation Regulation |
| ISSN号 | 1554-8627 |
| DOI | 10.1080/15548627.2016.1185576 |
| 通讯作者 | Liu, zhixue(zxliu@sibs.ac.cn) |
| 英文摘要 | Macroautophagy/autophagy is a conserved catabolic process that recycles cytoplasmic material during low energy conditions. becn1/beclin1 (beclin 1, autophagy related) is an essential protein for function of the class 3 phosphatidylinositol 3-kinase (ptdins3k) complexes that play a key role in autophagy nucleation and elongation. here, we show that amp-activated protein kinase (ampk) regulates autophagy by phosphorylating becn1 at thr388. phosphorylation of becn1 is required for autophagy upon glucose withdrawal. becn1(t388a), a phosphorylation defective mutant, suppresses autophagy through decreasing the interaction between pik3c3 (phosphatidylinositol 3-kinase catalytic subunit type 3) and atg14 (autophagy-related 14). the becn1(t388a) mutant has a higher affinity for bcl2 than its wild-type counterpart; the mutant is more prone to dimer formation. conversely, a becn1 phosphorylation mimic mutant, t388d, has stronger binding to pik3c3 and atg14, and promotes higher autophagy activity than the wild-type control. these findings uncover a novel mechanism of autophagy regulation. |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; ENDOPLASMIC-RETICULUM STRESS ; STARVATION-INDUCED AUTOPHAGY ; BECLIN 1 PHOSPHORYLATION ; DAP-KINASE ; CELL-DEATH ; ULK1 ; INDUCTION ; BCL-2 ; TUMORIGENESIS |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000384022000004 |
| 出版者 | TAYLOR & FRANCIS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375889 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Zhixue |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Nutr & Metab, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Shanghai, Peoples R China 3.Tianjin Haihe Hosp, Tianjin Inst Resp Dis, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Deyi,Wang, Wei,Sun, Xiujie,et al. Ampk regulates autophagy by phosphorylating becn1 at threonine 388[J]. Autophagy,2016,12(9):1447-1459. |
| APA | Zhang, Deyi.,Wang, Wei.,Sun, Xiujie.,Xu, Daqian.,Wang, Chenyao.,...&Liu, Zhixue.(2016).Ampk regulates autophagy by phosphorylating becn1 at threonine 388.Autophagy,12(9),1447-1459. |
| MLA | Zhang, Deyi,et al."Ampk regulates autophagy by phosphorylating becn1 at threonine 388".Autophagy 12.9(2016):1447-1459. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。