Epigallocatechin gallate inhibits hbv dna synthesis in a viral replication - inducible cell line
文献类型：期刊论文
| 作者 | He, Wei; Li, Li-Xia; Liao, Qing-Jiao; Liu, Chun-Lan; Chen, Xu-Lin |
| 刊名 | World journal of gastroenterology
 |
| 出版日期 | 2011-03-21 |
| 卷号 | 17期号:11页码:1507-1514 |
| 关键词 | Covalently closed circular dna Epigallocatechin gallate Hepatitis b virus e antigen Hepatitis b virus Precore mrna Replicative intermediates of dna |
| ISSN号 | 1007-9327 |
| DOI | 10.3748/wjg.v17.i11.1507 |
| 通讯作者 | Chen, xu-lin(chenxl@wh.iov.cn) |
| 英文摘要 | Aim: to analyze the antiviral mechanism of epigallocatechin gallate (egcg) against hepatitis b virus (hbv) replication. methods: in this research, the hbv-replicating cell line hepg2.117 was used to investigate the antiviral mechanism of egcg. cytotoxicity of egcg was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. hepatitis b virus e antigen (hbeag) and hepatitis b virus surface antigen (hbsag) in the supernatant were detected by enzyme-linked immunosorbent assay. precore mrna and pregenomic rna (pgrna) levels were determined by semi-quantitative reverse transcription polymerase chain reaction (pcr) assay. the effect of egcg on hbv core promoter activity was measured by dual luciferase reporter assay. hbv covalently closed circular dna and replicative intermediates of dna were quantified by real-time pcr assay. results: when hepg2.117 cells were grown in the presence of egcg, the expression of hbeag was suppressed, however, the expression of hbsag was not affected. hbv precore mrna level was also down-regulated by egcg, while the transcription of precore mrna was not impaired. the synthesis of both hbv covalently closed circular dna and replicative intermediates of dna were reduced by egcg treatment to a similar extent, however, hbv pgrna transcripted from chromosome-integrated hbv genome was not affected by egcg treatment, indicating that egcg targets only replicative intermediates of dna synthesis. conclusion: in hepg2.117 cells, egcg inhibits hbv replication by impairing hbv replicative intermediates of dna synthesis and such inhibition results in reduced production of hbv covalently closed circular dna. (c) 2011 baishideng. all rights reserved. |
| WOS关键词 | HEPATITIS-B-VIRUS ; GREEN TEA CATECHINS ; IN-VITRO ; GENE-EXPRESSION ; PROSTATE-CANCER ; INFECTION ; CCCDNA ; (-)-EPIGALLOCATECHIN-3-GALLATE ; CHEMOPREVENTION ; POLYPHENOLS |
| WOS研究方向 | Gastroenterology & Hepatology |
| WOS类目 | Gastroenterology & Hepatology |
| 语种 | 英语 |
| WOS记录号 | WOS:000289380500017 |
| 出版者 | BAISHIDENG PUBLISHING GROUP INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375891 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Xu-Lin |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Wei,Li, Li-Xia,Liao, Qing-Jiao,et al. Epigallocatechin gallate inhibits hbv dna synthesis in a viral replication - inducible cell line[J]. World journal of gastroenterology,2011,17(11):1507-1514. |
| APA | He, Wei,Li, Li-Xia,Liao, Qing-Jiao,Liu, Chun-Lan,&Chen, Xu-Lin.(2011).Epigallocatechin gallate inhibits hbv dna synthesis in a viral replication - inducible cell line.World journal of gastroenterology,17(11),1507-1514. |
| MLA | He, Wei,et al."Epigallocatechin gallate inhibits hbv dna synthesis in a viral replication - inducible cell line".World journal of gastroenterology 17.11(2011):1507-1514. |
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来源：武汉病毒研究所
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