Evaluation of a real-time rt-pcr assay using minor groove binding probe for specific detection of chinese wild-type classical swine fever virus
文献类型：期刊论文
| 作者 | Wen, Guoyuan1,3; Zhang, Tao2,5; Yang, Jun1; Luo, Qingping1; Liao, Yonghong1; Hu, Zhibin4; Zhang, Rongrong1; Wang, Hongling1; Ai, Diyun1; Luo, Ling1 |
| 刊名 | Journal of virological methods
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 176期号:1-2页码:96-102 |
| 关键词 | Classical swine fever virus Real-time rt-pcr Differential diagnosis Wild-type strain Hclv strain |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2011.06.014 |
| 通讯作者 | Shao, huabin(shb1961@yahoo.com.cn) |
| 英文摘要 | A one-step real-time rt-pcr assay using a minor groove binding probe was developed for the specific detection of chinese wild-type classical swine fever virus (csfv). the assay detected wild-type csfv strains representing different genotypes, but did not amplify viral rna from the hog cholera lipinized virus (hclv) vaccine-strain and other porcine viruses. the assay had a detection limit of 10 copies/reaction or 3.0 median tissue culture infective dose/reaction. in comparison to the sequencing nested rt-pcr assay, the sensitivity and specificity of the assay were 98.3% and 94.3%. respectively, when testing 515 veterinary samples. wild-type csfv rna was detected in nasal swabs 2-4 days before detection in serum samples from pigs exposed to infection by contact. and 2-4 days prior to the onset of clinical disease. hclv rna remained undetectable in nasal swabs and serum samples from vaccinated pigs. in conclusion, the novel assay described in this study provides a rapid and sensitive method for differentiating between wild-type and the hclv-strain of csfv. it could be used for monitoring in csf outbreak areas or as a screening method for csfv eradication strategies. (c) 2011 elsevier b.v. all rights reserved. |
| WOS关键词 | HOG-CHOLERA VIRUS ; POLYMERASE-CHAIN-REACTION ; REVERSE-TRANSCRIPTION ; DIAGNOSTIC-TECHNIQUES ; DNA PROBES ; DISEASE ; VACCINE ; BINDER ; DIFFERENTIATION ; AMPLIFICATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000294033700014 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375892 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shao, Huabin |
| 作者单位 | 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430070, Peoples R China 4.Hubei Prov Anim Dis Control Ctr, Wuhan 430064, Peoples R China 5.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Guoyuan,Zhang, Tao,Yang, Jun,et al. Evaluation of a real-time rt-pcr assay using minor groove binding probe for specific detection of chinese wild-type classical swine fever virus[J]. Journal of virological methods,2011,176(1-2):96-102. |
| APA | Wen, Guoyuan.,Zhang, Tao.,Yang, Jun.,Luo, Qingping.,Liao, Yonghong.,...&Shao, Huabin.(2011).Evaluation of a real-time rt-pcr assay using minor groove binding probe for specific detection of chinese wild-type classical swine fever virus.Journal of virological methods,176(1-2),96-102. |
| MLA | Wen, Guoyuan,et al."Evaluation of a real-time rt-pcr assay using minor groove binding probe for specific detection of chinese wild-type classical swine fever virus".Journal of virological methods 176.1-2(2011):96-102. |
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来源：武汉病毒研究所
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