Detection of zika virus by sybr green one-step real-time rt-pcr
文献类型：期刊论文
| 作者 | Xu, Ming-Yue1,2; Liu, Si Qing1; Deng, Cheng-Lin1; Zhang, Qiu-Yan1,2; Zhang, Bo1 |
| 刊名 | Journal of virological methods
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 236页码:93-97 |
| 关键词 | Zika virus Real-time rt-pcr Sybr green |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2016.07.014 |
| 通讯作者 | Zhang, bo(zhangbo@wh.iov.cn) |
| 英文摘要 | The ongoing zika virus (zikv) outbreak has rapidly spread to new areas of americas, which were the first transmissions outside its traditional endemic areas in africa and asia. due to the link with newborn defects and neurological disorder, numerous infected cases throughout the world and various mosquito vectors, the virus has been considered to be an international public health emergency. in the present study, we developed a sybr green based one-step real-time rt-pcr assay for rapid detection of zikv. our results revealed that the real-time assay is highly specific and sensitive in detection of zikv in cell samples. importantly, the replication of zikv at different time points in infected cells could be rapidly monitored by the real-time rt-pcr assay. specifically, the real-time rt-pcr showed acceptable performance in measurement of infectious zikv rna. this assay could detect zikv at a titer as low as 1 pfu/ml. the real-time rt-pcr assay could be a useful tool for further virology surveillance and diagnosis of zikv. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | JAPANESE ENCEPHALITIS-VIRUS ; AEDES-AEGYPTI ; FEVER VIRUS ; DENGUE ; TRANSMISSION ; NS2B ; RNA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000383941900015 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375907 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Bo |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Ming-Yue,Liu, Si Qing,Deng, Cheng-Lin,et al. Detection of zika virus by sybr green one-step real-time rt-pcr[J]. Journal of virological methods,2016,236:93-97. |
| APA | Xu, Ming-Yue,Liu, Si Qing,Deng, Cheng-Lin,Zhang, Qiu-Yan,&Zhang, Bo.(2016).Detection of zika virus by sybr green one-step real-time rt-pcr.Journal of virological methods,236,93-97. |
| MLA | Xu, Ming-Yue,et al."Detection of zika virus by sybr green one-step real-time rt-pcr".Journal of virological methods 236(2016):93-97. |
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来源：中国科学院大学
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