Aptamer beacons for visualization of endogenous protein hiv-1 reverse transcriptase in living cells
文献类型：期刊论文
| 作者 | Liang, Yu1,2,3; Zhang, Zhiping1; Wei, Hongping1; Hu, Qinxue1; Deng, Jiaoyu1; Guo, Deyin2,3; Cui, Zongqiang1; Zhang, Xian-En1 |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2011-10-15 |
| 卷号 | 28期号:1页码:270-276 |
| 关键词 | Visualization Endogenous protein Hiv-1 reverse transcriptase Aptamer beacons Living cells |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2011.07.031 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) |
| 英文摘要 | Direct visualization of endogenous proteins in living cells remains a challenge. aptamer beacon is a promising technique to resolve this problem by combining the excellent protein binding specificity of the aptamer with the sensitive signal transduction mechanism of the molecular beacon. in this study, aptamer 93del against hiv-1 reverse transcriptase (rt) was engineered into aptamer beacons to recognize and image hiv-1 rt. the constructed aptamer beacons could specifically bind to hiv-1 ri and the beacon-rt binding showed effective fluorescence signal transduction in homogeneous solution. in solutions with 1 mu 4 of the aptamer beacon, the effective fluorescence signal increased with increasing concentration of hiv-1 ri from 0.5 mu m to 5 mu m. when the aptamer beacons were delivered into the living cells that transiently expressed hiv-1 rt, hiv-1 ri could be specifically labeled and imaged. the designed aptamer beacons were further successfully applied for ri imaging in hiv-1 integrated u1 cells. the method developed here may be extended to visualize many other endogenous proteins in living cells using appropriate aptamer beacons. (c) 2011 elsevier b.v. all rights reserved. |
| WOS关键词 | MESSENGER-RNA ; MOLECULAR BEACONS ; IN-VITRO ; STREPTOLYSIN-O ; LIGANDS ; OLIGONUCLEOTIDES ; PERMEABILIZATION ; HYBRIDIZATION ; SELECTION ; DELIVERY |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000295661700041 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375918 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Modern Virol Res Ctr, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yu,Zhang, Zhiping,Wei, Hongping,et al. Aptamer beacons for visualization of endogenous protein hiv-1 reverse transcriptase in living cells[J]. Biosensors & bioelectronics,2011,28(1):270-276. |
| APA | Liang, Yu.,Zhang, Zhiping.,Wei, Hongping.,Hu, Qinxue.,Deng, Jiaoyu.,...&Zhang, Xian-En.(2011).Aptamer beacons for visualization of endogenous protein hiv-1 reverse transcriptase in living cells.Biosensors & bioelectronics,28(1),270-276. |
| MLA | Liang, Yu,et al."Aptamer beacons for visualization of endogenous protein hiv-1 reverse transcriptase in living cells".Biosensors & bioelectronics 28.1(2011):270-276. |
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来源：武汉病毒研究所
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