Functional investigation of grass carp reovirus nonstructural protein ns80
文献类型：期刊论文
| 作者 | Cai, Lin; Sun, Xiaoyun; Shao, Ling; Fang, Qin |
| 刊名 | Virology journal
 |
| 出版日期 | 2011-04-14 |
| 卷号 | 8页码:10 |
| ISSN号 | 1743-422X |
| DOI | 10.1186/1743-422x-8-168 |
| 通讯作者 | Fang, qin(qfang@wh.iov.cn) |
| 英文摘要 | Background: grass carp reovirus (gcrv), a highly virulent agent of aquatic animals, has an eleven segmented dsrna genome encased in a multilayered capsid shell, which encodes twelve proteins including seven structural proteins (vp1-vp7), and five nonstructural proteins (ns80, ns38, ns31, ns26, and ns16). it has been suggested that the protein ns80 plays an important role in the viral replication cycle that is similar to that of its homologous protein mu ns in the genus of orthoreovirus. results: as a step to understanding the basis of the part played by ns80 in gcrv replication and particle assembly, we used the yeast two-hybrid (y2h) system to identify ns80 interactions with proteins ns38, vp4, and vp6 as well as ns80 and ns38 self-interactions, while no interactions appeared in the four protein pairs ns38-vp4, ns38-vp6, vp4-vp4, and vp4-vp6. bioinformatic analyses of ns80 with its corresponding proteins were performed with all currently available homologous protein sequences in arvs (avian reoviruses) and mrvs (mammalian reoviruses) to predict further potential functional domains of ns80 that are related to vfls (viral factory-like structures) formation and other roles in viral replication. two conserved regions spanning from aa (amino acid) residues of 388 to 433, and 562 to 580 were discovered in this study. the second conserved region with corresponding conserved residues tyr565, his569, cys571, asn573, and glu576 located between the two coiled-coils regions (aa similar to 513-550 and aa similar to 615-690) in carboxyl-proximal terminus were supposed to be essential to form vfls, so that aa residues ranging from 513 to 742 of ns80 was inferred to be the smallest region that is necessary for forming vfls. the function of the first conserved region including ala395, gly419, asp421, pro422, leu438, and leu443 residues is unclear, but one-third of the amino-terminal region might be species specific, dominating interactions with other viral components. conclusions: our results in this study together with those from previous investigations indicate the protein ns80 might play a central role in vfls formation and viral components recruitment in gcrv particle assembly, similar to the mu ns protein in arvs and mrvs. |
| WOS关键词 | FACTORY-LIKE INCLUSIONS ; MU-NS ; SIGMA-NS ; CORE PARTICLES ; TRIPHOSPHATASE ACTIVITIES ; SEQUENCE ALIGNMENT ; AMINO-TERMINUS ; M1 GENE ; AQUAREOVIRUS ; IDENTIFICATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | BIOMED CENTRAL LTD |
| WOS记录号 | WOS:000290945100001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375958 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Fang, Qin |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Lin,Sun, Xiaoyun,Shao, Ling,et al. Functional investigation of grass carp reovirus nonstructural protein ns80[J]. Virology journal,2011,8:10. |
| APA | Cai, Lin,Sun, Xiaoyun,Shao, Ling,&Fang, Qin.(2011).Functional investigation of grass carp reovirus nonstructural protein ns80.Virology journal,8,10. |
| MLA | Cai, Lin,et al."Functional investigation of grass carp reovirus nonstructural protein ns80".Virology journal 8(2011):10. |
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来源：武汉病毒研究所
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