Genetic diversity of novel circular ssdna viruses in bats in china
文献类型：期刊论文
| 作者 | Ge, Xingyi1; Li, Jialu1; Peng, Cheng1; Wu, Lijun1; Yang, Xinglou1; Wu, Yongquan1; Zhang, Yunzhi2; Shi, Zhengli1 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2011-11-01 |
| 卷号 | 92页码:2646-2653 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir.0.034108-0 |
| 通讯作者 | Shi, zhengli(zlshi@wh.iov.cn) |
| 英文摘要 | Novel circular ssdna genomes have recently been detected in animals and in the environment using metagenomic and high-throughput sequencing approaches. in this study, five full-length circular ssdna genomes were recovered from bat faecal samples using inverse pcr with sequences designed based on circovirus-related sequences obtained from solexa sequencing data derived from a random amplification method. these five sequences shared a similar genomic organization to circovirus or the recently proposed cyclovirus of the family circoviridae. the newly obtained circovirus/cyclovirus-like genomes ranged from 1 741 to 21 77 bp, and each consisted of two major orfs, orf1 and orf2, encoding putative replicase (rep) and capsid (cap) proteins, respectively. the potential stem-loop region was predicted in all five genomes, and three of them had the typical conserved nonanucleotide motif of cycloviruses. a set of primers targeting the conserved rep region was designed and used to detect the prevalence of circovirus/cyclovirus sequences in individual bats. among 199 samples tested, 47 were positive (23.6 %) for the circovirus genome and two (1.0 %) were positive for the cyclovirus genome. in total, 48 partial rep sequences plus the five full-length genomes were obtained in this study. detailed analysis indicated that these sequences are distantly related to known circovirus/cyclovirus genomes and may represent 22 novel species that belong to the family circoviridae. |
| WOS关键词 | CHICKEN ANEMIA VIRUS ; MULTISYSTEMIC WASTING SYNDROME ; PORCINE CIRCOVIRUS ; METAGENOMIC ANALYSIS ; FEATHER DISEASE ; GENOME ; IDENTIFICATION ; DNA |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000296952300020 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375961 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shi, Zhengli |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China 2.Yunnan Inst Endem Dis Control & Prevent, Dali, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Xingyi,Li, Jialu,Peng, Cheng,et al. Genetic diversity of novel circular ssdna viruses in bats in china[J]. Journal of general virology,2011,92:2646-2653. |
| APA | Ge, Xingyi.,Li, Jialu.,Peng, Cheng.,Wu, Lijun.,Yang, Xinglou.,...&Shi, Zhengli.(2011).Genetic diversity of novel circular ssdna viruses in bats in china.Journal of general virology,92,2646-2653. |
| MLA | Ge, Xingyi,et al."Genetic diversity of novel circular ssdna viruses in bats in china".Journal of general virology 92(2011):2646-2653. |
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来源：武汉病毒研究所
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