Isolation and characterization of a novel alphanodavirus
文献类型：期刊论文
| 作者 | Bai, Huimin1,2; Wang, Yun1; Li, Xiang1,4; Mao, Haitao1,5; Li, Yan1,2; Han, Shili3; Shi, Zhengli1; Chen, Xinwen1 |
| 刊名 | Virology journal
 |
| 出版日期 | 2011-06-19 |
| 卷号 | 8页码:7 |
| 关键词 | Alphanodavirus Hznv Hz-am1 Host susceptibility |
| ISSN号 | 1743-422X |
| DOI | 10.1186/1743-422x-8-311 |
| 通讯作者 | Chen, xinwen(chenxw@pentium.whiov.ac.cn) |
| 英文摘要 | Background: nodaviridae is a family of non-enveloped isometric viruses with bipartite positive-sense rna genomes. the nodaviridae family consists of two genera: alpha-and beta-nodavirus. alphanodaviruses usually infect insect cells. some commercially available insect cell lines have been latently infected by alphanodaviruses. results: a non-enveloped small virus of approximately 30 nm in diameter was discovered co-existing with a recombinant helicoverpa armigera single nucleopolyhedrovirus (hearnpv) in hz-am1 cells. genome sequencing and phylogenetic assays indicate that this novel virus belongs to the genus of alphanodavirus in the family nodaviridae and was designated hznv. hznv possesses a rna genome that contains two segments. rna1 is 3038 nt long and encodes a 110 kda viral protein termed protein a. the 1404 nt long rna2 encodes a 44 kda protein, which exhibits a high homology with coat protein precursors of other alphanodaviruses. hznv virions were located in the cytoplasm, in association with cytoplasmic membrane structures. the host susceptibility test demonstrated that hznv was able to infect various cell lines ranging from insect cells to mammalian cells. however, only hz-am1 appeared to be fully permissive for hznv, as the mature viral coat protein essential for hznv particle formation was limited to hz-am1 cells. conclusion: a novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in hz-am1 cells. |
| WOS关键词 | BLACK BEETLE VIRUS ; NODAMURA VIRUS ; MITOCHONDRIAL LOCALIZATION ; NODAVIRUS PARTICLES ; RNA REPLICATION ; PROTEIN-A ; CELL-LINE ; INSECT ; GENOME ; LEPIDOPTERA |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000292934800001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375963 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Xinwen |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Huazhong Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China 4.Wenzhou Med Coll, Dept Cell Biol, Wenzhou 325035, Peoples R China 5.Chinese Acad Sci, Inst Pasteur Shanghai, Viral Hepatitis Res Unit, Shanghai 200025, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Huimin,Wang, Yun,Li, Xiang,et al. Isolation and characterization of a novel alphanodavirus[J]. Virology journal,2011,8:7. |
| APA | Bai, Huimin.,Wang, Yun.,Li, Xiang.,Mao, Haitao.,Li, Yan.,...&Chen, Xinwen.(2011).Isolation and characterization of a novel alphanodavirus.Virology journal,8,7. |
| MLA | Bai, Huimin,et al."Isolation and characterization of a novel alphanodavirus".Virology journal 8(2011):7. |
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来源：武汉病毒研究所
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