Implication of the env gene of the human endogenous retrovirus w family in the expression of bdnf and drd3 and development of recent-onset schizophrenia
文献类型：期刊论文
| 作者 | Huang, WenJie1,2; Li, Shan1; Hu, YuanMing3; Yu, Honglian1; Luo, Feng4; Zhang, Qi5; Zhu, Fan1 |
| 刊名 | Schizophrenia bulletin
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 37期号:5页码:988-1000 |
| ISSN号 | 0586-7614 |
| 关键词 | Schizophrenia Herv-w Env Human u251 glioma cells Drd3 Bdnf Sirna |
| DOI | 10.1093/schbul/sbp166 |
| 通讯作者 | Zhu, fan(zhufan@hotmail.com) |
| 英文摘要 | Objective: retrovirus has been suggested as one of agents involved in the development of schizophrenia. in the present study, we examined the role of the human endogenous retrovirus w family (herv-w) env gene in the etiopathogenesis of recent-onset schizophrenia, using molecular and epidemiological approaches. methods: nested rt-pcr was used to detect the messenger rna (mrna) of the herv-w env gene in plasmas. quantitative real-time polymerase chain reaction (pcr) was employed to detect the viral reverse transcriptase activity in human sera. human u251 glioma cells were used to study the potential role of the herv-w env gene in the etiopathogenesis of recent-onset schizophrenia. results: we identified genes with mrna sequences homologous to herv-w env gene from plasmas of 42 out of 118 individuals with recent-onset schizophrenia but not from any of 106 normal persons (p < .01, t test). quantitative real-time pcr showed a significantly increase in the reverse transcriptase activity in the sera of patients (by 35.59%) compared with controls (by 2.83%) (p < .05, t test). overexpression of herv-w env in human u251 glioma cells upregulated brain-derived neurotrophic factor (bdnf), an important schizophrenia-associated gene, neurotrophic tyrosine kinase receptor type 2 (ntrk2, also called trkb), and dopamine receptor d3 and increased the phosphorylation of cyclic adenosine monophosphate response element-binding (creb) protein. bdnf promoter reporter gene assays showed that the herv-w env triggers bdnf production in human u251 glioma cells. using gene knockdown, we found that creb is required for the expression of bdnf that is regulated by env. conclusion: our data revealed that the transcriptional activation of herv is associated with the development of schizophrenia in some patients and indicated that herv-w env regulates the expression of schizophrenia-associated genes. this report is the first to elucidate the signaling pathway responsible for the upregulation of herv-w env-triggered bdnf. our study provides new evidence for the involvement of herv-w in the central nervous system, which will benefit the diagnosis and treatment of the devastating schizophrenia and related disorders. |
| WOS关键词 | NEUROTROPHIC FACTOR ; MULTIPLE-SCLEROSIS ; TOXOPLASMA-GONDII ; HERV-W ; RECEPTOR ; ACTIVATION ; CELLS ; RNA ; INDIVIDUALS ; MODEL |
| WOS研究方向 | Psychiatry |
| WOS类目 | Psychiatry |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000294557100018 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375970 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhu, Fan |
| 作者单位 | 1.Wuhan Univ, Sch Med, Dept Med Microbiol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 3.Wuhan Univ, Sch Math & Stat, Wuhan 430071, Peoples R China 4.Jianghan Univ, Sch Med, Wuhan, Peoples R China 5.Stanford Univ, Sch Med, Stanford, CA 94305 USA |
| 推荐引用方式 GB/T 7714 | Huang, WenJie,Li, Shan,Hu, YuanMing,et al. Implication of the env gene of the human endogenous retrovirus w family in the expression of bdnf and drd3 and development of recent-onset schizophrenia[J]. Schizophrenia bulletin,2011,37(5):988-1000. |
| APA | Huang, WenJie.,Li, Shan.,Hu, YuanMing.,Yu, Honglian.,Luo, Feng.,...&Zhu, Fan.(2011).Implication of the env gene of the human endogenous retrovirus w family in the expression of bdnf and drd3 and development of recent-onset schizophrenia.Schizophrenia bulletin,37(5),988-1000. |
| MLA | Huang, WenJie,et al."Implication of the env gene of the human endogenous retrovirus w family in the expression of bdnf and drd3 and development of recent-onset schizophrenia".Schizophrenia bulletin 37.5(2011):988-1000. |
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来源：武汉病毒研究所
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