A novel luciferase and gfp dual reporter virus for rapid and convenient evaluation of hepatitis c virus replication
文献类型：期刊论文
| 作者 | Wu, Yang; Liao, Qingjiao; Yang, Rongge; Chen, Xinwen; Chen, Xulin |
| 刊名 | Virus research
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 155期号:2页码:406-414 |
| 关键词 | Hepatitis c virus Renilla luciferase Green fluorescent protein Reporter gene Interferon Ribavirin |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2010.11.009 |
| 通讯作者 | Chen, xulin(chenxl@wh.iov.cn) |
| 英文摘要 | Herein, we describe the development of a monocistronic dual reporter virus for monitoring hepatitis c virus (hcv) replication. the recombinant construct encodes for the humanized renilla luciferase (hrluc) reporter gene inserted upstream of the viral open reading frame and a green fluorescent protein (gfp) gene inserted into the c-terminus of non-structural protein 5a (ns5a) of the jfh1 viral genome. the viral rna replicated efficiently in transfected cells and infectious virions could be produced without obvious attenuation of viral replication. the viral titer of the dual reporter virus was comparable to that of single reporter viruses. the expression levels of these two reporter genes correlated well with hcv replication in the presence or absence of antiviral agents. moreover, because of the direct visibility of gfp fluorescence and the correlation between gfp positive cell numbers and hrluc activity, the optimal time for measuring hrluc activity was determined. this novel infectious system is a time saving and cost effective method for studying the interaction between viruses and host cells as well as for screening anti-hcv drugs. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | RNA REPLICATION ; VIRAL GENOME ; INFECTION ; INSERTION ; CULTURE ; SYSTEM |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000287544400004 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375975 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Xulin |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yang,Liao, Qingjiao,Yang, Rongge,et al. A novel luciferase and gfp dual reporter virus for rapid and convenient evaluation of hepatitis c virus replication[J]. Virus research,2011,155(2):406-414. |
| APA | Wu, Yang,Liao, Qingjiao,Yang, Rongge,Chen, Xinwen,&Chen, Xulin.(2011).A novel luciferase and gfp dual reporter virus for rapid and convenient evaluation of hepatitis c virus replication.Virus research,155(2),406-414. |
| MLA | Wu, Yang,et al."A novel luciferase and gfp dual reporter virus for rapid and convenient evaluation of hepatitis c virus replication".Virus research 155.2(2011):406-414. |
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来源：武汉病毒研究所
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