Hiv-1 vpr increases hcv replication through vprbp in cell culture
文献类型：期刊论文
| 作者 | Yan, Yanling1,2; Huang, Fang1; Yuan, Ting1; Sun, Binlian1; Yang, Rongge1 |
| 刊名 | Virus research
 |
| 出版日期 | 2016-09-02 |
| 卷号 | 223页码:153-160 |
| 关键词 | Hiv-1 Hcv Vpr Vprbp Cullin ring e3 ligases |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2016.07.007 |
| 通讯作者 | Sun, binlian(sunbl@wh.iov.cn) ; Yang, rongge(ryang@wh.iov.cn) |
| 英文摘要 | Coinfection of human immunodeficiency virus (hiv) and hepatitis c virus (hcv) occurs at a high frequency, in which hiv shows a promotion of hcv-derived liver diseases. however, the mechanism of how this occurs is not well understood. our previous work has demonstrated that the hiv-1 accessory protein vpr enhances hcv rna replication in cell culture. because vpr performs most of its functions through host protein vprbp (dcaf1), the role of vprbp in the regulation of hcv by vpr was investigated in this study. we found that the vpr mutant q65r, which is deficient in vprbp binding, could not enhance hcv replication. furthermore, vpr-mediated enhancement of hcv replication was severely diminished in vprbp knockdown cells. in addition, an inhibitor of cullin ring e3 ligases, mln4924, impaired the function of vpr during hcv replication. together, these results suggest that vpr promotes hcv replication in a vprbp-dependent manner, and that the activity of cullin ring e3 ligases is essential to this process. in conclusion, our findings demonstrate that hiv-1 vpr makes the cellular environment more suitable for hcv replication, which might relate with the host ubiquitination system. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | HEPATITIS-C-VIRUS ; HUMAN-IMMUNODEFICIENCY-VIRUS ; TELANGIECTASIA MUTATED KINASE ; E3 UBIQUITIN LIGASE ; TYPE-1 VPR ; CYCLE ARREST ; PROTEIN VPR ; NUCLEAR-LOCALIZATION ; RNA REPLICATION ; INFECTION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000383826600020 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375986 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, Binlian; Yang, Rongge |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Mol Virol, Res Grp HIV Mol Epidemiol & Virol,State Key Lab V, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Yanling,Huang, Fang,Yuan, Ting,et al. Hiv-1 vpr increases hcv replication through vprbp in cell culture[J]. Virus research,2016,223:153-160. |
| APA | Yan, Yanling,Huang, Fang,Yuan, Ting,Sun, Binlian,&Yang, Rongge.(2016).Hiv-1 vpr increases hcv replication through vprbp in cell culture.Virus research,223,153-160. |
| MLA | Yan, Yanling,et al."Hiv-1 vpr increases hcv replication through vprbp in cell culture".Virus research 223(2016):153-160. |
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来源：中国科学院大学
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