Efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna
文献类型：期刊论文
| 作者 | Zhang, Yi1,2; Liang, Zhen1,2; Zong, Yuan1,2; Wang, Yanpeng1,2; Liu, Jinxing1; Chen, Kunling1; Qiu, Jin-Long3; Gao, Caixia1 |
| 刊名 | Nature communications
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 7页码:8 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/ncomms12617 |
| 通讯作者 | Gao, caixia(cxgao@genetics.ac.cn) |
| 英文摘要 | Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing crispr/cas9 introduced as dna or rna. this transient expression-based genome-editing system is highly efficient and specific for producing transgene-free and homozygous wheat mutants in the t0 generation. we demonstrate our protocol to edit genes in hexaploid bread wheat and tetraploid durum wheat, and show that we are able to generate mutants with no detectable transgenes. our methods may be applicable to other plant species, thus offering the potential to accelerate basic and applied plant genome-engineering research. |
| WOS关键词 | NATURAL VARIATION ; CAS9 NUCLEASE ; COMMON WHEAT ; CROP PLANTS ; HUMAN-CELLS ; RICE ; SYSTEM ; GENE ; RIBONUCLEOPROTEINS ; SPECIFICITY |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000383663700001 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2375990 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, Caixia |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yi,Liang, Zhen,Zong, Yuan,et al. Efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna[J]. Nature communications,2016,7:8. |
| APA | Zhang, Yi.,Liang, Zhen.,Zong, Yuan.,Wang, Yanpeng.,Liu, Jinxing.,...&Gao, Caixia.(2016).Efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna.Nature communications,7,8. |
| MLA | Zhang, Yi,et al."Efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna".Nature communications 7(2016):8. |
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来源：中国科学院大学
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