Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome
文献类型：期刊论文
| 作者 | Li, You1; Wang, Shuai1; Zhu, Hua2; Zheng, Chunfu1 |
| 刊名 | Archives of virology
 |
| 出版日期 | 2011-12-01 |
| 卷号 | 156期号:12页码:2267-2272 |
| ISSN号 | 0304-8608 |
| DOI | 10.1007/s00705-011-1094-9 |
| 通讯作者 | Zheng, chunfu(zheng.alan@hotmail.com) |
| 英文摘要 | Herpes simplex virus type 1 (hsv-1) is a ubiquitous human pathogen of skin and mucous membranes. in the present study, the genome of the hsv-1 f strain was cloned as an infectious bacterial artificial chromosome (bac) clone without any deletions of the viral genes. additionally, a firefly luciferase cassette was inserted to generate a novel luciferase-expressing hsv-1 bac. importantly, the resulting recombinant hsv-1 bac luc behaved indistinguishably from the wild-type virus in vero cells, and the luciferase activity could be easily quantified in vitro. thus, this novel hsv-1 bac system would serve as a powerful tool for gene function profiling. |
| WOS关键词 | IN-VIVO ; ESCHERICHIA-COLI ; CONSTRUCTION ; MUTAGENESIS ; BAC ; DNA ; GENERATION ; MUTANTS ; REVEALS ; SYSTEM |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000297706400018 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376016 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Chunfu |
| 作者单位 | 1.Chinese Acad Sci, Mol Virol & Viral Immunol Res Grp, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.UMDNJ New Jersey Med Sch, Dept Microbiol & Mol Genet, Newark, NJ 07101 USA |
| 推荐引用方式 GB/T 7714 | Li, You,Wang, Shuai,Zhu, Hua,et al. Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome[J]. Archives of virology,2011,156(12):2267-2272. |
| APA | Li, You,Wang, Shuai,Zhu, Hua,&Zheng, Chunfu.(2011).Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome.Archives of virology,156(12),2267-2272. |
| MLA | Li, You,et al."Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome".Archives of virology 156.12(2011):2267-2272. |
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来源：武汉病毒研究所
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