Cra negatively regulates acid survival in yersinia pseudotuberculosis
文献类型：期刊论文
| 作者 | Hu, Yangbo1,2; Lu, Pei1,2; Zhang, Yong1; Li, Yunlong1,2; Li, Lamei1,2; Huang, Li1,2; Chen, Shiyun1 |
| 刊名 | Fems microbiology letters
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 317期号:2页码:190-195 |
| 关键词 | Cra Acid survival Yersinia pseudotuberculosis Proteomic analysis Carbohydrate metabolism |
| ISSN号 | 0378-1097 |
| DOI | 10.1111/j.1574-6968.2011.02227.x |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) |
| 英文摘要 | Survival in acidic environments is important for successful infection of gastrointestinal pathogens. many bacteria have evolved elaborate mechanisms by inducing or repressing gene expression, which subsequently provide ph homeostasis and enable acid survival. in this study, we employed comparative proteomic analysis to identify the acid-responsive proteins of a food-borne enteric bacterium, yersinia pseudotuberculosis. the expression level of eight proteins involved in carbohydrate metabolism was up- or downregulated over twofold at ph 4.5 compared with ph 7.0. the role of a global transcriptional regulator catabolite repressor/activator cra was further studied in this acid survival process. lacz-fusion analysis showed that expression of cra was repressed under acidic ph. deletion of the cra gene increased acid survival by 10-fold, whereas complementation restored the wild-type phenotype. these results lead us to propose that, in response to acidic ph, the expression of cra gene is downregulated to increase acid survival. this is the first study to demonstrate the regulatory role of cra in acid survival in an enteric bacterium. |
| WOS关键词 | ESCHERICHIA-COLI ; SALMONELLA-TYPHIMURIUM ; GENE-EXPRESSION ; TOLERANCE RESPONSE ; SHIGELLA-FLEXNERI ; PH RESPONSE ; RESISTANCE ; VIRULENCE ; SYSTEM ; CATABOLISM |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000288703700010 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376022 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Shiyun |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yangbo,Lu, Pei,Zhang, Yong,et al. Cra negatively regulates acid survival in yersinia pseudotuberculosis[J]. Fems microbiology letters,2011,317(2):190-195. |
| APA | Hu, Yangbo.,Lu, Pei.,Zhang, Yong.,Li, Yunlong.,Li, Lamei.,...&Chen, Shiyun.(2011).Cra negatively regulates acid survival in yersinia pseudotuberculosis.Fems microbiology letters,317(2),190-195. |
| MLA | Hu, Yangbo,et al."Cra negatively regulates acid survival in yersinia pseudotuberculosis".Fems microbiology letters 317.2(2011):190-195. |
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来源：武汉病毒研究所
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