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Analysis of the ecdysteroid udp-glucosyltransferase gene of heliothis armigera single-nucleocapsid baculovirus

文献类型:期刊论文

作者Chen, XW; Hu, ZH; Jehle, JA; Zhang, YQ; Vlak, JM
刊名Virus genes
出版日期1997
卷号15期号:3页码:219-225
关键词Baculovirus Heliothis armigera egt gene Sequence analysis Phylogeny
ISSN号0920-8569
通讯作者Chen, xw()
英文摘要An ecdysteroid udp-glucosyltransferase (egt) gene was identified from the single (s) nucleocapsid nucleopolyhedrovirus of heliothis armigera (hearnpv). in baculovirus-infected insects the viral enzyme (egt) plays a pivotal role in abrogating the insect molting process, the open reading frame of the egt gene is 1545 nucleotides long, encoding a putative protein of 515 amino acids with a m-r of 59.1. the 5'-noncoding region contains a putative early (cagt) and late (taag) motif for transcription initiation, a transcription enhancer sequence (cgtcgc) and two tata boxes. a putative polya signal, aataaa, was found downstream of the translation stop codon, a putative signal peptide of 21 residues was present at the n-terminus of the egt, the hearnpv egt gene has a high degree of nucleotide and amino acid sequence homology to the egt genes of buzura suppressaria snpv and spodoptera exigua mnpv. the hearnpv egt shares ten conserved motifs with other egts. a phylogenetic tree of twelve baculovirus egts was constructed by using maximum parsimony analysis, suggesting that snpvs do not form a separate clade within the baculovirus family.
WOS关键词NUCLEAR POLYHEDROSIS-VIRUS ; NUCLEOTIDE-SEQUENCE ; IDENTIFICATION
WOS研究方向Genetics & Heredity ; Virology
WOS类目Genetics & Heredity ; Virology
语种英语
WOS记录号WOS:000071781400004
出版者KLUWER ACADEMIC PUBL
URI标识http://www.irgrid.ac.cn/handle/1471x/2376027
专题武汉病毒研究所
通讯作者Chen, XW
作者单位1.Acad Sinica, Wuhan Inst Virol, Wuhan 430071, Peoples R China
2.Staatliche Lehrund Forsch Anstalt, D-67435 Neustadt, Germany
3.Agr Univ Wageningen, Dept Virol, NL-6709 PD Wageningen, Netherlands
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GB/T 7714
Chen, XW,Hu, ZH,Jehle, JA,et al. Analysis of the ecdysteroid udp-glucosyltransferase gene of heliothis armigera single-nucleocapsid baculovirus[J]. Virus genes,1997,15(3):219-225.
APA Chen, XW,Hu, ZH,Jehle, JA,Zhang, YQ,&Vlak, JM.(1997).Analysis of the ecdysteroid udp-glucosyltransferase gene of heliothis armigera single-nucleocapsid baculovirus.Virus genes,15(3),219-225.
MLA Chen, XW,et al."Analysis of the ecdysteroid udp-glucosyltransferase gene of heliothis armigera single-nucleocapsid baculovirus".Virus genes 15.3(1997):219-225.

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来源:武汉病毒研究所

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